Skip to main content

Table 2 Identification of glycosylated proteins, peptides and their glycosylation sites in royal jelly proteins

From: Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly

Accession

Glycoprotein and glycopeptide

-10lgP

Mass(Da)

Charge

No. of spectra

Amino acid residue no.

Glycosylation site (N-type)

Enrichment method

Lectin

Hydrazide

        

Orbitrap

Triple TOF

Orbitrap

Triple TOF

gi|58585098

Major royal jelly protein 1 precursor

442.09

48886

        
 

R.GESLN(+2.99)KSLPILHEWK.Fb

57.82

585.32

3

8

24-38

28

√

 

√

 

gi|58585108

Major royal jelly protein 2 precursor

398.06

51074

        
 

K.TSNHLKQIEIPHDIAVN(+2.99)ATTGK.Ga

67.92

797.42

3

15

162-183

178

√

√

√

√

 

K.IAIDKFDRLWVLDSGLVN(+2.99)R.Ta

57.57

1117.1

2

9

128-146

145

√

√

√

√

gi|284182838

Major royal jelly protein 4

298.73

53015

        
 

R.KN(+2.99)LTNTLNVIHEWK.Yb

57.71

856.97

2

5

30-41

31

√

 

√

 
 

K.M(+15.99)SNQQEN(+2.99)LTLKEVDNK.Vb

47.23

637.31

3

2

236-251

242

√

 

√

 

gi|58585188

Major royal jelly protein 6 precursor

203.5

49786

        
 

K.NYPFDVDQWHN(+2.99)K.Td

47.63

783.35

2

4

68-79

78

√

 

√

 
 

K.LLAFDLN(+2.99)TSKLLK.Qd

47

739.94

2

2

158-170

164

 

√

  

gi|62198227

Major royal jelly protein 7 precursor

312.92

50541

        
 

K.QVDIPHEIAVN(+2.99)TTTEQGR.Ld

54.49

1006

2

11

168-185

178

√

√

√

√

 

R.LWVLDSGLVN(+2.99)NTQPM(+15.99)C(+57.02)FPK.Ld

45.55

1112

2

8

136-154

145

√

√

√

 
 

K.NGILFFGLVN(+2.99)NTAVGC(+57.02)WNEHQ(+0.98)TLQ(+0.98)R.Ed

57.66

1447.2

2

2

312-316

321

  

√

 

gi|67010041

Major royal jelly protein 9 precursor

107.71

48688

        
 

K.IPHDIAIN(+2.99)STTGK.Rd

40.14

685.37

2

2

170-182

177

√

   

gi|148277624

Yellow-e3 precursor

89

48235

        
 

K.YM(+15.99)SGTLNSN(+2.99)ETNFR.Ie

50.32

826.86

2

2

384-397

392

  

√

 

gi|328787887

Lysosomal alpha-mannosidase

165.89

188194

        
 

R.LTQSFHYYEGM(+15.99)EGNNM(+15.99)EFKN(+2.99)R.Sd

53.93

877.38

3

4

538-558

557

√

 

√

 
 

R.LLKDDAFGVGEALN(+2.99)ESAYGEGLVVR.Gd

50.85

1313.2

2

2

722-746

735

√

   

gi|89885579

Alpha-glucosidase

193.65

65565

        
 

K.N(+2.99)VSRDSN(+2.99)SSDFKK.Lb

39.74

497.24

3

2

313-325

313 319

√

   
 

K.HM(+15.99)LIEAYTN(+2.99)LSM(+15.99)TM(+15.99)K.Yb

38.65

917.42

2

2

282-296

290

 

√

  

gi|66564326

Plasma glutamate carboxypeptidase isoform 1

178.08

52947

        
 

K.ESADYGLENVHGEN(+2.99)VTVPFWVR.Gd

63.89

1261.1

2

2

99-120

112

√

   
 

R.SVTPYSLYTPHTGHQSYGEN(+2.99)VTK.Id

55.98

643.06

4

5

212-234

231

√

 

√

 
 

R.IM(+15.99)TLLSPM(+15.99)GN(+2.99)LTVR.Sd

42.7

790.92

2

3

394-407

403

√

√

  
 

R.AIM(+15.99)NEALN(+2.99)GSFK.Gd

34.47

657.33

2

2

54-65

61

 

√

  

gi|328778095

Lysosomal Pro-X carboxypeptidase

71.61

56432

        
 

R.YYGESM(+15.99)PYNN(+2.99)K.Se

35.71

692.79

2

1

124-134

133

  

√

 

gi|66560290

Lysosomal aspartic protease

62.1

42222

        
 

K.N(+2.99)GTDFAIR.Ye

35.21

448.72

2

1

120-127

120

√

   

gi|328782027

Membrane metallo-endopeptidase 1

89.03

88720

        
 

K.HNPIPDN(+.98)KVEWSEDEIKAN(+2.99)K.Te

50.01

592.55

4

2

92-111

110

√

   
 

K.WYDN(+.98)SGVN(+2.99)TSTAK.Ie

37.35

723.83

2

1

311-323

318

√

   
 

R.IVNTN(+2.99)DTETR.Le

36.04

583.28

2

1

31-40

35

√

   

gi|48118838

Glucosylceramidase

142.8

58571

        
 

K.QFDNN(+2.99)ITYLKEEHYETYVNYLIK.Fd

54.42

735.86

4

3

211-233

215

√

 

√

 
 

K.N(+2.99)FSLAPEDYNYK.Id

46.71

732.33

2

2

171-182

171

√

   
 

K.TQANWIANYFGPILASSPFN(+2.99)K.Td

30.3

781.41

3

1

273-293

292

 

√

  
 

R.M(+15.99)N(+2.99)VSEVKFDR.Cd

40.85

622.3

2

3

72-81

73

√

√

√

 

gi|58585090

Glucose oxidase

277.69

67938

        
 

R.SNLHVIVN(+2.99)ATVTK.Vd

54.93

699.9

2

8

277-289

284

√

√

√

√

 

K.LVN(+2.99)TTVM(+15.99)RDLGVEFQK.Id

52.05

934.99

2

7

499-514

501

√

√

√

√

 

R.WVQQGAFGWSWDEVM(+15.99)PYYLKSEN(+2.99)NTELSR.Vd

40.6

1180.9

3

4

172-200

194

  

√

√

 

R.AFITPFEN(+2.99)R.Sd

41.19

549.28

2

3

268-276

275

  

√

√

 

K.YYTTN(+2.99)ESHACLSTGGSCYWPR.Gd

41.83

800.34

3

2

126-146

130

  

√

 

gi|166795901

Apolipophorin-III-like protein precursor

226.7

21348

        
 

K.DQSANFVNNIQDYIKN(+2.99)VTEEVK.Td

62.84

857.76

3

6

71-92

86

√

√

√

 

gi|328782084

Antithrombin-III

97.9

59502

        
 

K.ISN(+2.99)DSAQNGERDSIYHLIER.Ld

48.05

580.78

4

2

362-381

364

√

   

gi|187281543

Venom dipeptidyl peptidase 4 precursor

80.93

87937

        
 

R.HLAFATFN(+2.99)DTNVR.Dc

47.19

503.59

3

2

232-244

239

√

   
 

R.ANSFN(+2.99)GTWK.Tc

39.89

514.24

2

2

64-72

68

√

   
 

K.YSWIDSN(+2.99)R.Tc

34.35

522.24

2

2

625-632

631

  

√

 

gi|110755367

Toll-like receptor 13 isoform 1

127.33

75706

        
 

R.M(+15.99)LEHLDLSN(+2.99)NSLSTVNR.Rd

55.39

981.48

2

2

547-563

555

√

   
 

R.HLNTQFFHN(+2.99)TTNLNK.Ld

54.76

611.31

3

2

166-180

174

√

   
 

K.LHTLEEGLFAN(+2.99)LTR.Ld

50.96

539.62

3

2

432-445

442

√

   
 

R.LSEEAFKN(+2.99)ASK.Ld

39.92

613.81

2

1

315-325

322

√

   

gi|328790726

Venom acid phosphatase Acph-1

142.25

42665

        
 

K.M(+15.99)PSTINFYPNDPYIN(+2.99)YTYEPAGK.Gd

57.47

1357.6

2

2

38-60

52

√

   

gi|328792524

Hypothetical protein LOC408570

89.27

90763

        
 

R.WSLTPVNSN(+2.99)TTVVVK.Qe

43.11

824.45

2

2

539-553

547

√

   
 

R.QN(+2.99)YTDAPPAK.Le

41.13

554.27

2

2

590-599

591

√

   
 

R.IDPN(+2.99)SSFTQSNPIR.Fe

38.14

789.89

2

2

284-297

287

√

 

√

 

gi|66514614

Idgf4

135.8

48741

        
 

R.LKDLTIGVLPHVN(+2.99)STVYYDAR.Ld

57.43

595.07

4

2

216-236

228

√

   

gi|110766389

Protein takeout

90.9

30201

        
 

R.ALFSN(+2.99)ITVIGAGN(+2.99)YSLTK.Sd

56.15

938

3

2

105-122

109 117

√

 

√

 

gi|48094573

Hypothetical protein LOC408608

292.9

19434

        
 

K.GNLGTVN(+2.99)LTKVLKSVEDR.Ld

53.12

641.34

4

5

61-78

67

√

√

√

√

gi|110763647

Hypothetical protein LOC726323

109.47

18478

        
 

R.IYDPITN(+2.99)TSK.Md

35.5

577.8

2

1

133-142

139

  

√

 
  1. Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database of Apis (downloaded April 2012, version 4.5 of the honeybee genome). “-10logP” is the score calculated by PEAKS software (version 6.0, Bioinformatics Solutions Inc.). Charge is the number of the carrying charge of the peptide. No. of spectra is the number of the spectrum of the peptide generated by mass spectrometry. Amino acid residue No. corresponds to the position of the N-terminal and C-terminal amino acid of the peptide in the protein sequence. Glycosylation site indicates the position of the N-glycosylated amino acids of the peptide in the protein sequence. Orbitrap refers the peptides analyzed by the Q-Exactive mass spectrometry (Thermo Fisher Scientific). Triple TOF refers the peptides analyzed by Triple TOF 5600 (AB SCIEX). Lectin denotes N-glycopeptides enriched by the lectin method. Hydrazide represents N-glycopeptides enriched by hydrazide chemistry. “√” indicates that peptide is identified by the corresponding enrichment method and mass spectrometer. “a” is the known site in the known protein. “b” is the potential site (bioinformatics has predicted these potential sites in UniProt Database (updated April 2013), and it is experimentally confirmed in this study) in the known protein. “c” is the potential site in the novel protein. “d” denotes the novel site in the known protein. “e” is the novel site in the novel protein.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us