Relative copy number of genes located on the left and right arm of chromosome XV in BR-RF versus BR-S. Comparison of relative copy number was performed by qPCR using chromosomal DNA isolated from BR-RF and BR-S strains. Positions of examined genes on chromosome XV are marked by black arrows at Figure 5. Red, genes located at the left arm of the chromosome XV the expression of which is upregulated in BR-RF when compared with BR-S (and BR-F); blue, genes located at the right arm of the chromosome XV. Y axis, relative gene copy number (BR-RF versus BR-S); the exact values are stated below the ORFs. The mean of values from 3 independent experiments ± SD are shown; data significance (relative copy number of each left-arm-gene compared to relative copy number of each right-arm-gene) was determined using the two-tailed t test. All P values varied between P < 0.05 and P < 0.005.