Table 3 Novel miRNAs identified in the A line and B line of Brassica campestris by high-throughput sequencing
miR_name | miR_seq | LM | LP | CG% | dG | MFEI | FbA | FbB |
|---|---|---|---|---|---|---|---|---|
bra-miRn1-1-5p | CTATCGGTCTACTCGGTCAGC | 21 | 153 | 50 | -131.5 | 1.2 | 19 | 7 |
bra-miRn1-1-3p | TGACCGAGTAGACCGATAGTC | 21 | 153 | 50 | -131.5 | 1.2 | 87 | 222 |
bra-miRn1-2-5p | CTATCGGTCTACTCGGTCAGC | 21 | 153 | 47 | -152.2 | 1.3 | 19 | 7 |
bra-miRn1-2-3p | TGACCGAGTAGACCGATAGTC | 21 | 153 | 47 | -152.2 | 1.3 | 87 | 222 |
bra-miRn2-5p | CAACAGTCTCAGGATGGAAAA | 21 | 134 | 35 | -47.6 | 1 | 3 | 12 |
bra-miRn2-3p | TTTCATCTTAGAGAATGTTGTT | 22 | 134 | 35 | -47.6 | 1 | 57 | 153 |
bra-miRn3-5p | TACAAAGCTGAAGCTAATTATG | 22 | 141 | 41 | -70.2 | 0.9 | 18 | 56 |
bra-miRn3-3p | TAATCAGCTCCAGCTATGTACA | 22 | 141 | 41 | -70.2 | 0.9 | 145 | 175 |
bra-miRn4-5p | GAATGATACTTGGATATGATC | 21 | 147 | 34.7 | -80.3 | 1.5 | 14 | 6 |
bra-miRn4-3p | TTATATCCAAGTATCATTCCT | 21 | 147 | 34.7 | -80.3 | 1.5 | 15 | 34 |
bra-miRn5-5p | TTCTAAGCTTTACGGGAAACC | 21 | 201 | 29 | -101.4 | 1.7 | 10 | 11 |
bra-miRn5-3p | TTTCCCGTAAAGCTTAGAACC | 21 | 201 | 29 | -101.4 | 1.7 | 12 | 7 |
bra-miRn6-5p | GTCAATTGGTGATAGTAGTTC | 21 | 84 | 36.7 | -38.3 | 1.2 | 11 | 41 |
bra-miRn6-3p | TCTACTTTCACCAATTGGCCT | 21 | 84 | 36.7 | -38.3 | 1.2 | 4 | 54 |
bra-miRn7-5p | TTTTGCGTTTCAACTCGGTCC | 21 | 139 | 38.8 | -64 | 0.9 | 73 | 61 |
bra-miRn7-3p | GCTGAGTTGGAACACAAAATC | 21 | 139 | 38.8 | -64 | 0.9 | 19 | 8 |
bra-miRn8-5p | AGAGATGTCTGGCTTGCAACA | 21 | 140 | 44.5 | -74.3 | 1.1 | 1 | 3 |
bra-miRn8-3p | TTGCAAGCCAGACATTTCCTTT | 22 | 140 | 44.5 | -74.3 | 1.1 | 5 | 9 |
bra-miRn9-5p | TTTGGATTTTGGTCATTGTTG | 21 | 107 | 32.1 | -50.7 | 1.4 | 0 | 2 |
bra-miRn9-3p | ACAATGAACGAAATCCAAATC | 21 | 107 | 32.1 | -50.7 | 1.4 | 4 | 9 |
bra-miRn10-1-5p | ACAGGTGGTGGAACAAATATGAGT | 24 | 128 | 31.8 | -52.5 | 1.3 | 1 | 13 |
bra-miRn10-1-3p | TCATATTAGTTCTACCTCCTGCTG | 24 | 128 | 31.8 | -52.5 | 1.3 | 2 | 7 |
bra-miRn10-2-5p | ACAGGTGGTGGAACAAATATGAGT | 24 | 130 | 31.6 | -44.4 | 1 | 1 | 13 |
bra-miRn10-2-3p | TCATATTAGTTCTACCTCCTGCTG | 24 | 130 | 31.6 | -44.4 | 1 | 2 | 7 |
bra-miRn11-5p | TGAGTCTCTCACCAGTCTTTCAC | 23 | 117 | 34.1 | -59.3 | 1.4 | 2 | 2 |
bra-miRn11-3p | GAGAGACTCTGAAAGACTCACC | 22 | 117 | 34.1 | -59.3 | 1.4 | 8 | 5 |
bra-miRn12-5p | TGTAATTGCGGGGTTCTAAGC | 21 | 204 | 29.1 | -103.6 | 1.7 | 7 | 9 |
bra-miRn12-3p | TTAGAAACCTGCAATTATATA | 21 | 204 | 29.1 | -103.6 | 1.7 | 3 | 3 |
bra-miRn13-5p | ACTATGCAATTGTGAACAAAC | 21 | 128 | 29.5 | -56.4 | 1.3 | 2 | 3 |
bra-miRn13-3p | TTATTCACAACTGCATAATTC | 21 | 128 | 29.5 | -56.4 | 1.3 | 2 | 0 |
bra-miRn14-5p | GGGAGCCAGGGAAGAGGCAGT | 21 | 165 | 41.7 | -66 | 0.9 | 0 | 2 |
bra-miRn14-3p | TGCTTGTTCCCTGTCTCTCTC | 21 | 165 | 41.7 | -66 | 0.9 | 4 | 1 |
bra-miRn15-5p | ACCCGTCTCTTAATTTTTAAC | 21 | 161 | 31.7 | -59.4 | 1.1 | 19 | 32 |
bra-miRn15-3p | TAAAAGTTAAGAGACAAGTTA | 21 | 161 | 31.7 | -59.4 | 1.1 | 0 | 1 |
bra-miRn16-5p | ATAAAACGATTACACAGCTCGGTC | 24 | 230 | 42.1 | -209.8 | 2.1 | 1 | 1 |
bra-miRn16-3p | CGAGCTGTGTAATCGTTTTGTTA | 23 | 230 | 42.1 | -209.8 | 2.1 | 1 | 0 |
bra-miRn17-5p | TCTCGTTCTCTCGTTTCAGCT | 21 | 114 | 39.6 | -56.6 | 1 | 0 | 3 |
bra-miRn17-3p | CTGAAGCTAGTGAAAGAGAGA | 21 | 114 | 39.6 | -56.6 | 1 | 0 | 2 |
bra-miRn18-5p | TTGTTGACAAATACTTAGGCTC | 22 | 154 | 33.5 | -121.6 | 1.7 | 3 | 7 |
bra-miRn18-3p | GAGCCTAAGTATTTGTCAACAATG | 24 | 154 | 33.5 | -121.6 | 1.7 | 0 | 7 |
bra-miRn19-5p | TAAACAACACATATACTTTGC | 21 | 132 | 37 | -89.6 | 1.8 | 0 | 2 |
bra-miRn19-3p | AAACTATATGTGTTGCTTAGA | 21 | 132 | 37 | -89.6 | 1.8 | 1 | 0 |
bra-miRn20-5p | AAGAACTCGTCTCTTAACTTTTAA | 24 | 177 | 30.7 | -86.7 | 1.2 | 1 | 5 |
bra-miRn20-3p | AAACTAAGAGATGAATTCTTAC | 22 | 177 | 30.7 | -86.7 | 1.2 | 1 | 1 |
bra-miRn21-5p | NGCGGATATCTTAGGATGAGGT | 22 | 144 | 28.7 | -55.8 | 1.3 | 0 | 1 |
bra-miRn21-3p | TCATCGTAAGAGATCTGCATT | 21 | 144 | 28.7 | -55.8 | 1.3 | 0 | 1 |
bra-miRn22-5p | TGAGTTATCATTGGTCTTGTG | 21 | 186 | 28.1 | -94.6 | 1.8 | 0 | 1 |
bra-miRn22-3p | ACACAGGAACAATACTAACTCATT | 24 | 186 | 28.1 | -94.6 | 1.8 | 2664 | 4123 |
bra-miRn23-5p | CTTTGTCTATCGTTTGGAAAAG | 22 | 101 | 37.4 | -37 | 0.9 | 25 | 95 |
bra-miRn23-3p | TTTCCAAATGTAGACAAAGCT | 21 | 101 | 37.4 | -37 | 0.9 | 0 | 1 |