Table 2 Summary of EST-based SSRs identified

Total EST sequences examined

40,947

Total SSRs

2,405

   - Dinucleotide motif

750

   - Trinucleotide motif

1,591

   - Tetranucleotide motif

24

   - Pentanucleotide motif

12

   - Hexanucleotide motif

28

ESTs containing SSRs (SSR-ESTs)

2,214

   - ESTs containing 1 SSR

2,023

   - ESTs containing more than 2 SSRs

96

   - ESTs with SSRs in compound formation

95

SSR primer design

   - ESTs used for SSR primer design

937

   - ESTs excluded for primer design

    · exact match between both parents

740

    · short flanking sequence insufficient to design primers

624