Defining the AC16 transcriptome using GRO-Seq. A) Overview of the experimental scheme and treatments for the GRO-seq and ChIP-seq experiments in AC16 cells. B) Genome-browser view of the genomic region around the NFKB1 gene showing the distribution of GRO-seq reads, and Pol II and NF-κB p65 ChIP-seq reads in control and TNFα-treated AC16 cells at the indicated time points. C) Classification of all expressed transcripts in AC16 cells. Pie chart showing the composition of the AC16 transcriptome based on known and de novo annotations and functional assignments. D) Schematic representation of some of the transcript types listed in panel (C).