Figure 4

GRO-seq and Pol II ChIP-seq reveal the dynamics of TNFα-dependent transcription in AC16 cells. GRO-seq and ChIP-seq in AC16 cells treated with a time course of TNFα reveals the dynamics of transcription. A) Metagene representations showing the average GRO-seq read distributions ± 4 kb around the TSSs of upregulated (top) or downregulated (bottom) RefSeq genes over a time course of TNFα treatment in AC16 cells. B and C) Metagene representations showing the average Pol II (B) and NF-κB p65 (C) ChIP-seq read distributions ± 4 kb around the TSSs of upregulated (left) or downregulated (right) RefSeq genes at 0 and 30 min of TNFα treatment.