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Figure 1 | BMC Genomics

Figure 1

From: Functional genomics reveals that Clostridium difficileSpo0A coordinates sporulation, virulence and metabolism

Figure 1

Growth and sporulation kinetics of C. difficile 630 derivatives in broth cultures. a) Growth curves of the C. difficile 630 erm parental strain and its spo0A isogenic mutant. Shown are the OD600 measurements on the left y-axis and ethanol-resistant spore-derived CFUs on the right y-axis. The gray vertical arrow indicates that sampling point. b) Western blot analysis demonstrating that Spo0A is expressed under exponential and stationary growth stages by the parental C. difficile 630 erm strain but not the isogenic spo0A mutant. Spo0A-his6 protein was purified protein as described before [21].

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