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Table 8 Biotrophic gene Expression of putative Secreted Proteins

From: Genome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthora roreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phases

Transcript Id

Gene annotation

RNAseq

QPCR

  

30DPI

60DPI

Pvalue

Fold change

Primers

Induced 30-60 DPI

Repressed 30-60 DPI

  

Mean*

Mean*

   

Mean

St. E.

Mean

St. E.

e.m.s_0369_0002.2

Acetylxylan esterase

638.4

91.6

5.8E-03

7.0

     

e.m.s_0461_0001.1

Carbohydrate esterase family 8 protein

512.1

67.0

8.8E-04

7.6

MrCE8a

20.868

8.9736

  

e.m.s_0215_0001.21

Carbonic anhydrase

16.3

0.4

2.2E-02

      

e.m.s_0250_0003.8

Catalase cat1

294.9

59.3

6.4E-03

5.0

MrCAT1

114.906

48.407

  

e.m.s_0155_0004.13

Cell wall glycosyl hydrolase

330.4

26.3

7.4E-05

12.6

     

e.m.s_0192_0001.1

Cerato-platanin

3014.0

520.7

5.3E-03

5.8

     

e.m.s_0192_0003.14

Cerato-platanin

4931.3

968.5

7.2E-03

5.1

     

e.m.s_0192_0003.17

Cerato-platanin

7602.6

912.5

7.1E-04

8.3

     

e.m.s_0258_0001.1

Defense-related protein scp domain-containing protein MrPR-1 g

16778.8

1200.4

5.6E-04

14.0

MrSCP

434.018

253.661

  

e.m.s_0251_0004.1

Defense-related protein scp domain-containing protein MrPR-1i2

32.6

2.4

7.4E-02

      

e.m.s_0516_0001.1

Defense-related protein scp domain-containing protein Mr-PR-1n

499.0

13.7

2.6E-06

38.3

     

e.m.s_0041_0005.1

Endo-polygalacturonase

2621.4

32.4

1.4E-06

81.9

MrendoPG

911.453

592.910

  

e.m.s_0407_0004.2

Galactan 1,3-beta-galactosidase

37.6

52.2

2.4E-02

      

e.m.s_0027_0002.19

Galactose 1-epimerase

154.2

47.4

3.9E-02

3.3

     

e.m.s_0011_0002.91

Glycoside hydrolase family 16 protein

1276.8

232.0

5.0E-03

5.5

     

e.m.s_0030_0003.33

Glycoside hydrolase family 18 protein

6125.5

948.8

3.4E-03

6.5

MrGH18g

38.900

16.126

  

e.m.s_0044_0001.33

Glycoside hydrolase family 18 protein

527.3

56.3

7.7E-04

9.4

MrGH18d

253.832

183.817

  

e.m.s_0044_0001.6

Glycoside hydrolase family 18 protein

12287.0

3336.6

2.1E-02

3.7

     

e.m.s_0114_0002.6

Glycoside hydrolase family 18 protein

12684.6

687.6

5.1E-06

18.5

MrGH18c

151.387

51.810

  

e.m.s_0416_0001.5

Glycoside hydrolase family 18 protein

4897.1

266.4

3.1E-06

18.4

     

e.m.s_0055_0003.9

Glycoside hydrolase family 28 protein

129.2

20.9

1.3E-02

6.1

     

e.m.s_0067_0006.17

Glycoside hydrolase family 43

234.2

54.4

2.2E-02

4.3

     

e.m.s_0043_0001.2

Glycoside hydrolase family 5 protein

3971.1

188.7

1.2E-06

21.1

     

e.m.s_0531_0001.4

Glycoside hydrolase family 5 protein

3387.9

716.4

1.2E-02

4.7

MrGH5

69.7456

41.581

  

e.m.s_0351_0003.10

Glycoside hydrolase family 92 protein

2935.9

894.7

4.6E-02

3.3

     

e.m.s_0346_0003.2

Hydrophobin 3

431.5

65.6

1.8E-03

6.6

MrSc3P3

345.0789

196.349

  

e.m.s_0346_0003.1

Hydrophobin sc3-like

6461.8

471.4

3.8E-05

13.7

MrSc3P2

356.391

177.576

  

e.m.s_0409_0005.4

Hypothetical fad dependent oxidoreductase

566.2

119.8

1.4E-02

4.7

     

e.m.s_0561_0002.1

Immunomodulatory protein

7180.5

746.5

3.2E-04

9.6

     

e.m.s_0095_0005.5

Major facilitator superfamily multidrug-resistance

7.0

0.0

1.3E-02

      

e.m.s_0053_0004.6

Membrane autotransporter barrel domain protein

755.7

203.5

2.5E-02

3.7

MrBDP

5.184

1.528

  

e.m.s_0094_0001.8

O-methyltransferase

1131.7

488.6

8.2E-04

      

e.m.s_0064_0001.5

Tryptophan dimethylallyltransferase

190.8

0.9

3.2E-04

211.1

MrTRY-DMA

235.864

134.155

  

e.m.s_0039_0002.43

WSC domain protein

356.0

102.1

4.1E-02

3.5

     

e.m.s_0478_0002.2

Xylanase A

1135.8

9.0

9.3E-04

126.0

MrXYLa

171.086

77.159

  
  1. Mean* is the normalized mean based on the size factor of the libraries.