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Table 9 Necrotrophic genes expression of putative Secreted proteins

From: Genome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthora roreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phases

Transcript Id

Gene annotation

RNAseq

QPCR

  

30DPI

60DPI

Pvalue

Fold change

Primers

Induced 30-60 DPI

Repressed 30-60 DPI

  

Mean*

Mean*

   

Mean

St. E.

Mean

St. E.

e.m.s_0228_0004.4

Alpha beta hydrolase

3.2

34.5

1.4E-02

10.8

     

e.m.s_0245_0001.13

Alpha1,3-glucanase/mutanase

0.7

171.8

8.3E-03

245.4

     

e.m.s_0185_0001.10

Aryl-alcohol oxidase

0.0

21.4

5.7E-04

21.4

     

e.m.s_0185_0001.9

Aryl-alcohol oxidase

0.0

7.8

4.2E-02

7.8

     

e.m.s_0274_0001.9

Aryl-alcohol oxidase

0.0

9.6

2.1E-02

9.6

     

e.m.s_0491_0001.5

Aryl-alcohol oxidase

3.9

61.7

3.0E-02

15.9

     

e.m.s_0100_0003.8

Arylsulfatase

2.6

42.4

2.9E-02

16.4

     

e.m.s_0251_0003.2

Aspartic peptidase a1

11.5

46.7

3.6E-02

4

     

e.m.s_0056_0003.12

Aspartic-type endopeptidase

0.7

25.6

2.5E-03

36.6

     

e.m.s_0463_0001.8

Beta-lactamase class penicillin binding protein

6.6

324.6

1.5E-03

492.4

     

e.m.s_0066_0001.46

Biotrophy-associated secreted protein 2 MrBAS1

7.6

496.7

1.0E-03

66.1

MrSP2a

  

26.509

15.802

e.m.s_0005_0005.16

Biotrophy-associated secreted protein 2 MrBAS2

2.0

613.6

3.2E-04

322.6

     

e.m.s_0227_0001.20

Calcineurin-like phosphoesterase

1.8

19.0

9.5E-03

10.6

     

e.m.s_0066_0001.21

Carbohydrate esterase family 4 protein

0.7

83.8

1.8E-02

119.7

     

e.m.s_0155_0002.4

Carbohydrate esterase family 4 protein

0.0

50.5

2.1E-06

50.5

     

e.m.s_0008_0001.25

Carbonic anhydrase

0.7

121.3

7.0E-08

172.8

     

e.m.s_0190_0004.1

Carboxypeptidase cpds

3.8

460.0

4.4E-07

121.1

     

e.m.s_0190_0004.2

Carboxypeptidase cpds

3.1

28.5

5.1E-03

9.2

     

e.m.s_0176_0001.14

Cellulose-binding protein

0.0

22.2

4.7E-04

22.2

     

e.m.s_0058_0002.19

Cerato platanin

0.0

16.2

3.2E-03

16.2

     

e.m.s_0058_0002.21

Cerato-platanin

2.1

36.2

2.7E-02

18.1

     

e.m.s_0192_0001.2

Cerato-platanin

0.0

20.7

7.5E-04

20.7

     

e.m.s_0192_0001.6

Cerato-platanin

0.0

494.9

2.2E-04

494.9

     

e.m.s_0297_0002.3

Chitin deacetylase family 4

0.0

40.4

2.8E-02

40.5

MrCHIDACTb

  

5663.095

3460.583

e.m.s_0245_0001.24

Chitin synthase

0.0

2158.9

2.8E-04

2159

MrCHS

  

2515.177

1862.483

e.m.s_0008_0004.19

Copper amine oxidase

4.3

77.0

6.6E-03

17.9

     

e.m.s_0003_0001.85

Cutinase

0.0

9.5

2.3E-02

9.5

     

e.m.s_0017_0003.61

Cytochrome p450

0.0

32.2

9.1E-03

32.2

     

e.m.s_0052_0004.10

Cytochrome p450

0.7

14.9

3.2E-02

21.1

     

e.m.s_0470_0001.6

Cytochrome p450 monooxygenase

5.0

91.2

1.1E-04

18.3

MrCp450a

  

30.050

11.8905

e.m.s_0059_0001.14

Deuterolysin m35 metalloprotease

2.8

137.0

5.0E-02

48.9

     

e.m.s_0099_0003.13

Dioxygenase family protein

8.3

5904.3

3.6E-02

715.6

     

e.m.s_0061_0002.41

Exo-beta 1,3-glucanase

2.6

16.7

4.4E-02

6.4

     

e.m.s_0026_0005.53

Expansin family protein

0.0

1165.0

9.8E-04

1165

MrEXP-A

  

326.810

159.020

e.m.s_0086_0003.2

Expansin family protein

1.8

37.3

2.6E-04

20.7

     

e.m.s_0280_0002.5

Extracellular dioxygenase

0.0

15.1

3.4E-03

15.1

     

e.m.s_0107_0002.16

Extracellular triacylglycerol lipase precursor

0.0

9.8

2.0E-02

9.8

     

e.m.s_0107_0002.18

Extracellular triacylglycerol lipase precursor

0.0

9.8

2.3E-02

9.8

     

e.m.s_0352_0003.3

Extracellular triacylglycerol lipase precursor

0.0

9.7

2.1E-02

9.7

     

e.m.s_0054_0001.12

FAD binding domain protein

31.7

306.9

2.3E-04

9.7

     

e.m.s_0135_0001.27

FAD binding domain-containing protein

0.0

43.9

6.4E-06

43.9

     

e.m.s_0277_0002.12

FAD binding domain-containing protein

6.5

91.2

1.1E-04

14.1

     

e.m.s_0277_0002.13

FAD binding domain-containing protein

2.6

124.8

1.1E-04

48

     

e.m.s_0375_0001.9

FAD binding domain-containing protein

2.0

85.8

9.5E-06

43.1

     

e.m.s_0459_0001.6

FAD binding domain-containing protein

0.0

36.5

3.2E-04

36.5

     

e.m.s_0469_0001.4

FAD binding domain-containing protein

0.0

8.4

3.7E-02

8.4

     

e.m.s_0002_0010.20

F-box and fnip repeat-containing protein

114.0

3336.6

2.1E-02

29.3

     

e.m.s_0087_0002.1

Fruit-body specific gene a

0.7

20.9

7.7E-03

29.8

     

e.m.s_0053_0004.14

Fungal peroxidase

3.6

22.3

1.5E-02

6.3

     

e.m.s_0111_0006.5

Glucooligosaccharide oxidase

0.0

27.8

8.3E-03

27.8

     

e.m.s_0211_0001.14

Glucose oxidase

0.0

66.0

3.6E-07

66

     

e.m.s_0085_0002.37

Glycoside hydrolase family 16 protein

0.0

30.0

7.7E-05

29

     

e.m.s_0021_0002.67

Glycoside hydrolase family 18 protein

19.6

87.3

5.0E-02

4.5

     

e.m.s_0261_0004.2

Glycoside hydrolase family 3 protein

25.4

127.4

1.7E-02

5

     

e.m.s_0008_0004.3

Glycoside hydrolase family 30 protein

11.7

67.7

3.7E-02

6

     

e.m.s_0232_0001.2

Glycoside hydrolase family 35 protein

33.9

128.7

4.3E-02

3.8

     

e.m.s_0438_0002.3

Glycoside hydrolase family 35 protein

0.0

19.7

8.7E-04

19.7

     

e.m.s_0007_0001.127

Glycoside hydrolase family 5 protein

27.1

163.4

5.6E-03

6

     

e.m.s_0265_0002.8

Glycoside hydrolase family 5 protein

10.8

111.9

5.4E-05

10.4

     

e.m.s_0241_0001.11

Glycoside hydrolase family 61 protein

0.0

26.6

9.6E-03

26.6

     

e.m.s_0241_0001.12

Glycoside hydrolase family 61 protein

0.0

43.1

5.9E-03

43.1

     

e.m.s_0155_0002.8

Glycoside hydrolase family 61 protein

13.5

115.5

1.3E-02

8.6

     

e.m.s_0004_0003.50

Glycoside hydrolase family 9 protein

10.4

63.2

8.9E-03

6.3

MrGH9

  

4.311

1.827

e.m.s_0200_0003.3

Glycosyl hydrolase family 10

0.7

22.6

3.6E-02

32.2

     

e.m.s_0333_0001.1

Glyoxal oxidase

4.9

47.9

5.1E-03

9.8

     

e.m.s_0135_0001.28

gmc oxidoreductase

14.3

52.2

2.4E-02

3.6

     

e.m.s_0074_0003.10

Hemerythrin hhe cation binding domain-containing protein

49.9

383.1

2.1E-02

7.7

     

e.m.s_0039_0002.15

Hemolysin

0.7

16.1

2.3E-02

22.9

     

e.m.s_0062_0001.18

Heterokaryon incompatibility protein het-c

28.2

116.3

1.5E-02

4.1

     

e.m.s_0018_0008.40

Hydrophobin

13.2

86.6

3.6E-02

6.6

     

e.m.s_0018_0008.54

Hydrophobin

0.7

61.9

1.2E-05

87.1

     

e.m.s_0018_0008.56

Hydrophobin

0.0

256.2

4.3E-12

256

MrLM18

  

1573.702

1268.272

e.m.s_0058_0002.53

Hydrophobin

0.7

25.1

2.8E-03

35.7

     

e.m.s_0058_0002.55

Hydrophobin

9.0

304.7

5.8E-07

34.2

     

e.m.s_0149_0001.14

Hydrophobin

0.0

11.2

1.2E-02

11.2

     

e.m.s_0149_0001.16

Hydrophobin

0.0

425.5

2.3E-06

425.6

MrLM19

  

257.737

133.646

e.m.s_0170_0002.12

Hydrophobin

0.0

9.3

2.4E-02

9.3

     

e.m.s_0298_0001.2

Hydrophobin

0.0

11.4

1.2E-02

11.4

     

e.m.s_0149_0001.15

Hydrophobin 2

0.0

205.6

1.6E-04

205.6

     

e.m.s_0199_0001.3

Laccase

0.0

9.6

2.6E-02

9.6

     

e.m.s_0210_0002.8

Laccase

0.0

119.6

1.0E-06

119.6

     

e.m.s_0246_0002.7

Laccase

1.3

41.0

1.0E-02

31.8

     

e.m.s_0279_0002.3

Laccase

0.0

21.1

3.4E-02

21.2

     

e.m.s_0082_0001.16

Mannoprotein

88.8

298.1

4.7E-02

3.4

     

e.m.s_0030_0003.64

Metalloproteinase

18.6

306.9

8.1E-06

16.5

     

e.m.s_0406_0002.4

Necrosis inducing-like protein npp1 type

9.1

658.2

1.5E-04

73.1

MrNPP1

  

310.949

181.890

e.m.s_0051_0003.3

nhl repeat-containing protein

0.0

28.8

1.1E-04

28.8

     

e.m.s_0120_0002.7

Oxalate decarboxylase

9.3

57.9

4.8E-02

6.2

     

e.m.s_0398_0002.11

Oxidoreductase fad binding

1.8

111.0

5.4E-04

61.6

     

e.m.s_0639_0001.6

Para-nitrobenzyl esterase

0.0

45.8

3.2E-04

45.8

     

e.m.s_0082_0001.36

Peptidase m28

3.2

57.6

3.9E-02

18

     

e.m.s_0007_0001.138

Peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase a

0.0

12.6

3.5E-02

12.5

     

e.m.s_0180_0002.16

Phosphatidylserine decarboxylase

4.3

451.8

5.1E-04

105.1

     

e.m.s_0432_0001.5

PR-1 protein MrPR-1d

5.8

45.1

1.4E-03

7.8

     

e.m.s_0568_0001.7

Proline-rich antigen

7.7

1372.5

7.7E-03

177.3

     

e.m.s_0018_0003.1

Proline-specific peptidase

29.2

94.0

5.0E-02

3.2

     

e.m.s_0726_0001.2

Pyrolysin

8.1

50.9

3.5E-02

6.3

     

e.m.s_0125_0001.43

Riboflavin aldehyde-forming enzyme

61.2

229.9

3.8E-02

3.8

     

e.m.s_0040_0001.30

Ribonuclease t1

0.0

11.4

1.1E-02

11.4

     

e.m.s_0321_0001.8

Serine-rich protein

16.4

289.5

5.2E-04

18.1

MrSRP

  

14.072

5.613

e.m.s_0005_0005.47

Serine-type endopeptidase

224.0

811.1

4.1E-02

3.6

MrS-endo

  

3.482

0.866

e.m.s_0021_0002.36

Tripeptidyl peptidase A

0.0

20.8

6.5E-04

20.8

     

e.m.s_0166_0004.10

Tripeptidyl peptidase A

1.8

39.5

1.8E-04

21.9

     

e.m.s_0048_0003.5

Virus P4 KP4 toxin

0.0

118.9

4.5E-02

119

MrKP4

  

134.442

52.042

e.m.s_0014_0002.14

wsc domain-containing protein

0.0

42.6

8.7E-06

42.6

     

e.m.s_0010_0002.57

Zinc metalloprotease

10.7

92.8

2.6E-03

8.7

     
  1. Mean* is the normalized mean based on the size factor of the libraries.