Figure 5

Comparison of the sperm transcriptome with the available microarray datasets. (a) Venn diagram showing the overlap between the sperm transcriptome (in red) and the hermaphrodite germline gene datasets obtained by microarray analysis (in blue). The total number of genes in each dataset and the number of genes in the overlapping portion are indicated. (b) Venn diagram showing the overlap between the sperm transcriptome (in red) and the male germline gene datasets (in blue). (c) GO analysis of the male and hermaphrodite spermatogenesis-enriched genes (gray-shaded sets in (a) and (b)) shows that sperm are enriched in ion binding and kinase/phosphatase activities. (d) GO analysis of the sperm transcriptome shows that sperm are enriched in ion binding and phosphatase activities. (e) GO analysis of the sperm proteome shows that sperm are enriched in ion binding and kinase/phosphatase activities. **indicates P < 0.01 (hypergeometric probability test).