Figure 8

RNAi analysis of sperm genes. (a) Germline of the ogdh-1 RNAi worm. Yellow dashed line marks its immature germline. (b) asb-2 RNAi worm showed retarded growth of the germline (~60 h after egg stage). (c) Mock RNAi control (the same stage as (b)). (d) and (e) show the germlines of F22B5.4 and K09E4.1 RNAi worms. Yellow dashed lines mark the unfertilized oocytes. (f) Mock RNAi control. (g) and (h) show the brood sizes of the act-4, nspd-4, F22B5.4, and K09E4.1 RNAi worms are significantly reduced as compared with the control in which empty vector L4440 was used. *indicates P < 0.05 (Student’s t-test).