Comparative genomics of fully sequenced B. breve genomes and phylogenetic supertree. a) Venn diagram representing the orthologous and unique gene families as based on BLASTP comparison (E-value cut-off of 0.0001) and MCL clustering algorithm analyses. b) Cluster of Orthologues (COG) classification of the 1141 families of orthologues. For each COG entry the average percentage of hits among B. breve has been indicated. The most abundant families have also been indicated and they are assigned to housekeeping functions. From outer to inner circle: B. breve UCC2003, B. breve JCM 7017, B. breve 689b, B. breve NCFB 2258, B. breve S27, B. breve JCM 7019, B. breve 12L, B. breve ACS-071-V-Sch8b. c) Phylogenetic supertree showing the relationship between thirteen B. breve strains (B. breve UCC2003, B. breve 689b, B. breve 12L, B. breve NCFB 2258, B. breve S27, B. breve JCM 7017, B. breve JCM 7019, B. breve ACS-071-V-Sch8b, B. breve 2L, B. breve 31L, B. breve CECT 7263, B. breve DPC 6330, B. breve DSM 20213), B. longum subsp. longum NCC2705, B. longum subsp. infantis ATCC 15697, B. bifidum PRL2010, B. adolescentis ATCC 15703, B. dentium Bd1, B. animalis subsp. animalis ATCC 25527, B. animalis subsp. lactis DSM 10140 and B. asteroides PRL2011), three actinobacteria (G. vaginalis ATCC 14019, L. xyli subsp. xyli CTCB07 and T. whipplei TW08/27) and a single member of the Firmicutes as an outlier (Lb. plantarum WCFS1).