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Table 4 Results of qPCR validation of the expression of five miRNAs in MAC-T cells between treatments (challenged with E. coli or S. aureus bacteria) and controls at same time points

From: Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureusbacteria reveals pathogen directed microRNA expression profiles

Time points miRNA ID E. coli    S. aureus  
  Fold change* SE P-Value# Fold change SE P-Value
6h bta-miR-193a-3p 0.342 0.039 0.021 0.630 0.054 0.048
  bta-miR-21-3p 1.161 0.206 0.508 3.478 0.365 0.006
  bta-miR-365-3p 1.607 0.037 0.014 0.612 0.040 0.042
  bta-miR-423-5p 1.682 0.218 0.042 2.018 0.096 0.008
  bta-miR-486 2.109 0.304 0.036 1.616 0.046 0.008
12h bta-miR-193a-3p 0.992 0.023 0.911 0.601 0.085 0.010
  bta-miR-21-3p 2.154 0.276 0.017 1.062 0.272 0.819
  bta-miR-365-3p 0.930 0.115 0.568 0.994 0.106 0.962
  bta-miR-423-5p 0.629 0.046 0.004 0.812 0.106 0.111
  bta-miR-486 1.614 0.168 0.032 1.630 0.342 0.142
24h bta-miR-193a-3p 1.206 0.052 0.017 1.327 0.171 0.140
  bta-miR-21-3p 1.942 0.410 0.092 1.222 0.066 0.040
  bta-miR-365-3p 1.051 0.358 0.879 0.890 0.096 0.478
  bta-miR-423-5p 1.838 0.252 0.046 1.304 0.297 0.336
  bta-miR-486 1.099 0.030 0.545 0.867 0.119 0.480
48h bta-miR-193a-3p 0.353 0.053 0.020 0.336 0.026 0.021
  bta-miR-21-3p 1.083 0.192 0.679 1.105 0.080 0.385
  bta-miR-365-3p 1.612 0.067 0.006 2.809 0.204 0.008
  bta-miR-423-5p 1.092 0.017 0.130 2.894 0.258 0.010
  bta-miR-486 0.940 0.310 0.835 2.189 0.415 0.071
  1. *Fold change values are as compared to controls at same time points.
  2. #Bolded numbers indicate the significance with P < 0.05.