Table 4 Results of qPCR validation of the expression of five miRNAs in MAC-T cells between treatments (challenged with E. coli or S. aureus bacteria) and controls at same time points

6h

bta-miR-193a-3p

0.342

0.039

0.021

0.630

0.054

0.048

bta-miR-21-3p

1.161

0.206

0.508

3.478

0.365

0.006

bta-miR-365-3p

1.607

0.037

0.014

0.612

0.040

0.042

bta-miR-423-5p

1.682

0.218

0.042

2.018

0.096

0.008

bta-miR-486

2.109

0.304

0.036

1.616

0.046

0.008

12h

bta-miR-193a-3p

0.992

0.023

0.911

0.601

0.085

0.010

bta-miR-21-3p

2.154

0.276

0.017

1.062

0.272

0.819

bta-miR-365-3p

0.930

0.115

0.568

0.994

0.106

0.962

bta-miR-423-5p

0.629

0.046

0.004

0.812

0.106

0.111

bta-miR-486

1.614

0.168

0.032

1.630

0.342

0.142

24h

bta-miR-193a-3p

1.206

0.052

0.017

1.327

0.171

0.140

bta-miR-21-3p

1.942

0.410

0.092

1.222

0.066

0.040

bta-miR-365-3p

1.051

0.358

0.879

0.890

0.096

0.478

bta-miR-423-5p

1.838

0.252

0.046

1.304

0.297

0.336

bta-miR-486

1.099

0.030

0.545

0.867

0.119

0.480

48h

bta-miR-193a-3p

0.353

0.053

0.020

0.336

0.026

0.021

bta-miR-21-3p

1.083

0.192

0.679

1.105

0.080

0.385

bta-miR-365-3p

1.612

0.067

0.006

2.809

0.204

0.008

bta-miR-423-5p

1.092

0.017

0.130

2.894

0.258

0.010

bta-miR-486

0.940

0.310

0.835

2.189

0.415

0.071