Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles

Table 6 **KEGG (Kyoto encyclopedia of genes and genomes) pathways significantly enriched by target genes of 14 out of 17* differentially expressed miRNAs during E. coli or S. aureus challenge of MAC-T cells**

KEGG Pathways	miR-365-3p	miR-30b-5p/30c	Let-7a-5p	miR-23a	miR-148a	miR-24-3p	miR-92a	miR-193a-3p	miR-423-5p	miR-99b	miR-499	miR-486	miR-2339
Immune system
B cell receptor signaling pathway	X	X
T-cell receptor signaling		X	X
Fc gamma R-mediated, phagocytosis/chemokine signaling	X			X
Signal transduction
MAPK signaling pathway	X	X		X		X	X	X	X
ErbB signaling pathway		X	X		X	X
Wnt signaling pathway	X	X		X	X	X		X
TGF-beta signaling pathway			X	X	X	X
Calcium signaling pathway						X	X		X
Phosphatidylinositol pathway				X			X
ECM-receptor interaction			X		X
Cellular processes (transport and catabolism, cell motility, cell communication)
Endocytosis		X		X		X
Lysosome/apoptosis	X		X
Regulation of actin cytoskeleton	X	X		X	X	X	X				X
Cell cycle	X		X		X		X
P53 signaling pathway			X	X	X							X
Adherens/tight junction				X									X
Focal adhesion		X	X	X	X	X
Endocrine system
Insulin signaling pathway			X						X
Adipocytokine signaling pathway			X		X
GnRH signaling pathway			X					X	X
Melanogenesis	X					X				X
Nervous system
Long term potentiation	X	X					X			X
Neurotrophin signaling pathway	X	X	X	X		X		X
Development: Axon guidance		X	X	X	X	X			X		X	X	X
Metabolism: Glycerophospholipid metabolism/glycosphingolipid biosynthesis				X					X
Human diseases
Pathways in cancer		X	X	X	X			X	X	X	X	X
Colorectal cancer	X	X	X	X		X		X		X	X
Pancreatic cancer	X		X	X	X
Bladder cancer			X
Prostate cancer	X	X	X	X	X							X
Small cell lung cancer	X		X									X
Non-small cell lung cancer	X	X	X		X
Thyroid cancer			X
Renal cell carcinoma	X			X	X			X
Endometrical cancer	X		X
Glioma	X	X	X		X							X
Melanoma	X		X		X							X
Type II diabetes mellitus		X	X
Chronic myeloid leukemia	X		X	X	X
Acute myeloid leukemia					X			X

*Results for miR-21-3p were ignored because only few target genes out of 78 were non-significantly enriched in two pathways (Neurotophin signaling pathway- 3 genes and Ubiquitin mediated pathway- 3 genes). Similarly, only 3 genes each out of 88 target genes for miR-184 were enriched (P > 0.05) in two pathways, Chronic myeloid leukemia and Prostate cancer and were ignored. The target genes for miR-un5, a newly identified miRNA in this study were not determined.