Figure 7
Small-molecule chemical inhibitor treatments to identify which Ras1 downstream signaling pathways are involved in regulating DEGs in “pathways in cancer”, “insulin signaling pathway”, and “MAPK signaling pathway”. (A) Common DEGs in all the three pathways. (B) Common DEGs in “pathways in cancer” and “insulin signaling pathway”. (C) A common DEG in “pathways in cancer” and “MAPK signaling pathway”. (D) DEGs only in “pathways in cancer”. (E) DEGs only in “insulin signaling pathway”. (F) DEGs only in “MAPK signaling pathway”. Small-molecule chemical inhibitors of the Ras downstream effectors (Rafi, Raf inhibitor; LY294002, PI3K inhibitor; Rapa, rapamycin, TORC1 inhibitor; 15 μg/larva) are injected into Ras1CA-overexpressed silkworm larvae. Inhibitors are marked with different color: black, DMSO as a control; red: Raf inhibitor; green, LY294002; blue: rapamycin.