Small-molecule inhibitor treatments to identify which Ras1 downstream signaling pathways are involved in regulating DEGs in “purine metabolism” and “pyrimidine metabolism”. (A) Common DEGs in both pathways. (B) DEGs only in “purine metabolism”. Small-molecule chemical inhibitors of the Ras downstream effectors (Rafi, Raf inhibitor; LY294002, PI3K inhibitor; Rapa, rapamycin, TORC1 inhibitor; 15 μg/larva) are injected into Ras1CA-overexpressed silkworm larvae. Inhibitors are marked with different color: black, DMSO as a control; red: Raf inhibitor; green, LY294002; blue: rapamycin.