Figure 4

Extension of extradenticle sequence by meta-assembly. Contigs generated by multiple k-mer lengths were consolidated by meta-assembly to recover the entire coding sequence of the gene extradenticle from sequence fragments. Contigs from individual assemblies of multiple k-mer lengths are shown in alignment to the meta-assembly and the Drosophila transcript. The k-mer length 31 contigs were not included in the meta-assembly and show a reduction in coverage compared to other assemblies. Assemblies with shorter k-mer lengths also show a reduction in coverage but are not shown due to excessive fragmentation which results in a large number of short contigs that cannot be confidently aligned. The extended transcript aligns to the full length of the Drosophila reference sequence with 83% nucleotide sequence conservation.