Figure 7
From: Contribution of natural antisense transcription to an endogenous siRNA signature in human cells
Expression analysis of genes with short RNAs and their close neighbors. (A) Schematic representation of the investigated loci. (B) RT-qPCR analysis of mRNA levels in wt, clone 5 and clone 12. The values represent the ΔcT values as compared to β-actin. The genes producing the short RNAs are in bold. The assays for the genes not detectable in HEK293 cells (NLRP9 and GPR44) were tested positive using CaCo-2 cells which express these genes. All the standard deviations are below 1 PCR cycle.