Gene expression patterns across each of the four chromosomes of Fusarium graminearum . Each chromosome was divided into 20 kb windows. (A) Number of genes per window. The horizontal blue lines represent gene number of 6. (B) Gene expression levels per window. Log2-transformed read number per window was used to quantify gene expression levels. Twelve regions with low expression levels were identified on the four chromosomes, which matched non-conserved regions (except Nc8). The horizontal red lines represent an expression level of 10. (C) Number of non-conserved genes per window. All genes predicted in F. graminearum were matched with the genes of F. verticillioides using BLASTn tool and genes without homologs (p value > 1E-5) were selected. The number of these non-conserved genes per 20 kb window is shown in each chromosome of F. graminearum. (D) The number of non-expressed genes in each window of each chromosome is shown. Green boxes represent non-conserved regions in F. graminearum. The rDNA region on chromosome 4 is indicated by a blue dot.