Figure 7
Comparative overview of microarray and RT-qPCR analysis. Twenty five genes were identified by microarray technique as being primed by yeast G402 strain carrying either Ssa1, 2, 3 or 4 as sole source of Ssa family protein. Both microarray and RT-qPCR analysis was conducted using total RNA extracted from 5-ml cultures of the [psi-] yeast strains carrying Ssa1, 2, 3 or 4 grown overnight at 30°C. The 3D-GeneTM Yeast Oligo chip S.cerevisiae 6 k used for the microarray analysis according to the manufacturer ’s instruction. Relative mRNA expression levels were quantified relative to that of the housekeeping gene ACT1 (YFL039C) by 2^-∆∆Ct method [43]. Bothe the global normalize value of microarray and the relative mRNA expression levels were LOG-transformed to linearize the data and the heat map of was created using TIGR MultiExperiment Viewer (MeV) [41]. Results are based on two experiments, each with three replicates.