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Table 5 Primers and annealing temperatures for ompA and p-ompA

From: Gene polymorphism analysis of Yersinia enterocoliticaouter membrane protein A and putative outer membrane protein A family protein

Target gene

Primer direction

Primer Sequences (5′ → 3′)

GenBank no.

Location

Amplicon lengt

Annealing temp

ompA

Forward

ACATCACACTTGTAACTTTCTCACC

YP_001005874.1

1783285-1783261

1451 bp

58°C

 

Reverse

AGAAGTATCAGAATCAGATGTCGTC

 

1781835-1781859

  

p-ompA

Forward

GCGGCAAATTCCGTACAGTG

YP_001006877.1

2919405-2919386

1560 bp

60°C

 

Reverse

CAGCCCACCAGCAATATTCG

 

2917806-2917825

  
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