Table 5 Primers and annealing temperatures for ompA and p-ompA
| Target gene | Primer direction | Primer Sequences (5′ → 3′) | GenBank no. | Location | Amplicon lengt | Annealing temp |
|---|---|---|---|---|---|---|
| ompA | Forward | ACATCACACTTGTAACTTTCTCACC | YP_001005874.1 | 1783285-1783261 | 1451 bp | 58°C |
| Reverse | AGAAGTATCAGAATCAGATGTCGTC | 1781835-1781859 | ||||
| p-ompA | Forward | GCGGCAAATTCCGTACAGTG | YP_001006877.1 | 2919405-2919386 | 1560 bp | 60°C |
| Reverse | CAGCCCACCAGCAATATTCG | 2917806-2917825 |