Subcellular localization of selected BnaCPK-GFP fusion proteins in intact N. benthamiana leaf epidermal cells and protoplasts. Panels A-E represent the subcellular localizations of BnaCPK5-GFP, BnaCPK9-GFP, BnaCPK11-GFP, BnaCPK13-GFP and BnaCPK15-GFP, together with a plasma marker AtCBL1n fused with mCherry, respectively. A white arrow marks the region and direction in which the distribution of fluorescence intensities was scanned. The left panel is GFP fluorescence, the middle mCherry field and the right represents an overlay of the two images. PM, plasma membrane; nu, nuclei. Bar = 50 μm.