Yeast two-hybrid analysis of interactions between CPK and PP2C (Clade A) proteins in canola. The yeast cells of strain AH109 harboring the indicated plasmid combinations were grown on either the nonselective (SD-LW) or selective (SD-LWHA) media, followed by β-galactosidase assay (X-Gal staining). Decreasing cell densities in the dilution series are illustrated by narrowing triangles. Non-interacting BnaCPK-BnaPP2C or BnaCPK-BnaABF/AREB/ABI combinations were not shown here. AD is the empty pGADT7 vector.