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Table 1 Calcium-dependent protein kinase (CPK) genes identified and cloned from canola ( Brassica napus L.)

From: Identification, expression and interaction analyses of calcium-dependent protein kinase (CPK) genes in canola (Brassica napusL.)

Gene GenBank acc no. EST count Arabidopsis homolog/AGI no. Rice homolog/locusa CDS (bp) Protein M.W. (kDa) pI No. of EF-handsb N-termianal AA. Palmitoylation sitesc Myristoylation sitesd
BnaCPK1 JX122905 7 AtCPK1/At5g04870 OsCPK10/LOC_Os03g57450.1 1788 66.3 5.11 4 MGNTCVGP Yes n.d.
BnaCPK2 JX122906 4 AtCPK2/At3g10660 OsCPK10/LOC_Os03g57450.1 1854 68.8 5.27 4 MGNVCVGP Yes n.d.
BnaCPK3 JX122910 38 AtCPK3/At4g23650 OsCPK1/LOC_Os01g43410.1 1578 58.7 6.31 4 MGHRHSKS n.d. Yes
BnaCPK4 KF169735 2 AtCPK4/At4g09570 OsCPK24/LOC_Os11g07040.1 1503 56.3 5.12 4 MEKPSSRR n.d. n.d.
BnaCPK5 JX122911 30 AtCPK5/At4g35310 OsCPK13/LOC_Os04g49510.1 1638 61.3 5.47 4 MGNSCRRS Yes n.d.
BnaCPK6 JX122912 14 AtCPK6/At2g17290 OsCPK13/LOC_Os04g49510.1 1647 61.5 5.14 4 MGNSCRGS Yes n.d.
BnaCPK7 JX122913 15 AtCPK7/At5g12480 OsCPK20/LOC_Os07g38120.1 1599 59.5 5.97 4 MGNCCGSP Yes n.d.
BnaCPK8 JX122914 32 AtCPK8/At5g19450 OsCPK20/LOC_Os07g38120.1 1605 60.2 6.22 4 MGNCCASP Yes n.d.
BnaCPK9 JX122915 11 AtCPK9/At3g20410 OsCPK19/LOC_Os07g33110.1 1620 60.5 5.92 4 MGNCFAKN Yes Yes
BnaCPK10 JX122900 1 AtCPK10/At1g18890 OsCPK9/LOC_Os03g48270.1 1632 61.4 6.44 4 MGNCNVCV Yes n.d.
BnaCPK11 JX122901 9 AtCPK11/At1g35670 OsCPK24/LOC_Os11g07040.1 1494 56.1 5.17 4 MEKANPRR n.d. n.d.
BnaCPK12 JX122902 26 AtCPK12/At5g23580 OsCPK24/LOC_Os11g07040.1 1464 55.8 5.34 4 MANKSRTR n.d. n.d.
BnaCPK13 JX122903 24 AtCPK13/At3g51850 OsCPK3/LOC_Os01g61590.1 1587 59.5 6.49 4 MGNCCRSP Yes n.d.
BnaCPK15 JX122904 10 AtCPK15/At4g21940 OsCPK19/LOC_Os07g33110.1 1710 64.4 6.09 4 MGCFSSKH Yes Yes
BnaCPK17 KF169737 22 AtCPK17/At5g12180 OsCPK2/LOC_Os01g59360.1 1572 58.1 5.81 4 MGNCCSGR Yes Yes
BnaCPK18 KF169738 1 AtCPK18/At4g36070 OsCPK18/LOC_Os07g22710.1 1626 61.5 7.47 4 MGLCFSSP Yes Yes
BnaCPK21 JX122907 4 AtCPK21/At4g04720 OsCPK19/LOC_Os07g33110.1 1578 59.2 6.45 4 MGCLSSKH Yes Yes
BnaCPK24 KF169739 47 AtCPK24/At2g31500 OsCPK29/LOC_Os12g12860.1 1833 69.3 6.8 4 MGSCVSSP Yes Yes
BnaCPK28 JX122909 11 AtCPK28/At5g66210 OsCPK18/LOC_Os07g22710.1 1623 61 8.65 4 MGLCFSAI Yes Yes
BnaCPK29 JX122908 4 AtCPK29/At1g76040 OsCPK19/LOC_Os07g33110.1 1617 61 6.17 4 MDTRTKHF n.d. n.d.
BnaCPK30 KF169740 4 AtCPK30/At1g74740 OsCPK9/LOC_Os03g48270.1 1641 61.9 6.62 4 MGNCIACV Yes Yes
BnaCPK32 KF169741 4 AtCPK32/At3g57530 OsCPK20/LOC_Os07g38120.1 1617 60.9 6.17 4 MGNCCGTA Yes Yes
BnaCPK34 KC414030 10 AtCPK34/At5g19360 OsCPK2/LOC_Os01g59360.1 1458 54 5.28 4 MGNCCGRD Yes n.d.
  1. aInParanoid, http://inparanoid.sbc.su.se/cgi-bin/index.cgi. NA, InParanoid could not detect any orthologs for this gene.
  2. b The number of EF-hands were predicted by SMART scan and by sequence alignment.
  3. c The palmitoylation site were predicted by CSS-Palm 3.0.
  4. d The myristoylation sites were predicted by Myristoylator.
  5. n.d., not detected.
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BMC Genomics

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