Table 1 Calcium-dependent protein kinase (CPK) genes identified and cloned from canola ( Brassica napus L.)
Gene | GenBank acc no. | EST count | Arabidopsis homolog/AGI no. | Rice homolog/locusa | CDS (bp) | Protein M.W. (kDa) | pI | No. of EF-handsb | N-termianal AA. | Palmitoylation sitesc | Myristoylation sitesd |
|---|---|---|---|---|---|---|---|---|---|---|---|
BnaCPK1 | JX122905 | 7 | AtCPK1/At5g04870 | OsCPK10/LOC_Os03g57450.1 | 1788 | 66.3 | 5.11 | 4 | MGNTCVGP | Yes | n.d. |
BnaCPK2 | JX122906 | 4 | AtCPK2/At3g10660 | OsCPK10/LOC_Os03g57450.1 | 1854 | 68.8 | 5.27 | 4 | MGNVCVGP | Yes | n.d. |
BnaCPK3 | JX122910 | 38 | AtCPK3/At4g23650 | OsCPK1/LOC_Os01g43410.1 | 1578 | 58.7 | 6.31 | 4 | MGHRHSKS | n.d. | Yes |
BnaCPK4 | KF169735 | 2 | AtCPK4/At4g09570 | OsCPK24/LOC_Os11g07040.1 | 1503 | 56.3 | 5.12 | 4 | MEKPSSRR | n.d. | n.d. |
BnaCPK5 | JX122911 | 30 | AtCPK5/At4g35310 | OsCPK13/LOC_Os04g49510.1 | 1638 | 61.3 | 5.47 | 4 | MGNSCRRS | Yes | n.d. |
BnaCPK6 | JX122912 | 14 | AtCPK6/At2g17290 | OsCPK13/LOC_Os04g49510.1 | 1647 | 61.5 | 5.14 | 4 | MGNSCRGS | Yes | n.d. |
BnaCPK7 | JX122913 | 15 | AtCPK7/At5g12480 | OsCPK20/LOC_Os07g38120.1 | 1599 | 59.5 | 5.97 | 4 | MGNCCGSP | Yes | n.d. |
BnaCPK8 | JX122914 | 32 | AtCPK8/At5g19450 | OsCPK20/LOC_Os07g38120.1 | 1605 | 60.2 | 6.22 | 4 | MGNCCASP | Yes | n.d. |
BnaCPK9 | JX122915 | 11 | AtCPK9/At3g20410 | OsCPK19/LOC_Os07g33110.1 | 1620 | 60.5 | 5.92 | 4 | MGNCFAKN | Yes | Yes |
BnaCPK10 | JX122900 | 1 | AtCPK10/At1g18890 | OsCPK9/LOC_Os03g48270.1 | 1632 | 61.4 | 6.44 | 4 | MGNCNVCV | Yes | n.d. |
BnaCPK11 | JX122901 | 9 | AtCPK11/At1g35670 | OsCPK24/LOC_Os11g07040.1 | 1494 | 56.1 | 5.17 | 4 | MEKANPRR | n.d. | n.d. |
BnaCPK12 | JX122902 | 26 | AtCPK12/At5g23580 | OsCPK24/LOC_Os11g07040.1 | 1464 | 55.8 | 5.34 | 4 | MANKSRTR | n.d. | n.d. |
BnaCPK13 | JX122903 | 24 | AtCPK13/At3g51850 | OsCPK3/LOC_Os01g61590.1 | 1587 | 59.5 | 6.49 | 4 | MGNCCRSP | Yes | n.d. |
BnaCPK15 | JX122904 | 10 | AtCPK15/At4g21940 | OsCPK19/LOC_Os07g33110.1 | 1710 | 64.4 | 6.09 | 4 | MGCFSSKH | Yes | Yes |
BnaCPK17 | KF169737 | 22 | AtCPK17/At5g12180 | OsCPK2/LOC_Os01g59360.1 | 1572 | 58.1 | 5.81 | 4 | MGNCCSGR | Yes | Yes |
BnaCPK18 | KF169738 | 1 | AtCPK18/At4g36070 | OsCPK18/LOC_Os07g22710.1 | 1626 | 61.5 | 7.47 | 4 | MGLCFSSP | Yes | Yes |
BnaCPK21 | JX122907 | 4 | AtCPK21/At4g04720 | OsCPK19/LOC_Os07g33110.1 | 1578 | 59.2 | 6.45 | 4 | MGCLSSKH | Yes | Yes |
BnaCPK24 | KF169739 | 47 | AtCPK24/At2g31500 | OsCPK29/LOC_Os12g12860.1 | 1833 | 69.3 | 6.8 | 4 | MGSCVSSP | Yes | Yes |
BnaCPK28 | JX122909 | 11 | AtCPK28/At5g66210 | OsCPK18/LOC_Os07g22710.1 | 1623 | 61 | 8.65 | 4 | MGLCFSAI | Yes | Yes |
BnaCPK29 | JX122908 | 4 | AtCPK29/At1g76040 | OsCPK19/LOC_Os07g33110.1 | 1617 | 61 | 6.17 | 4 | MDTRTKHF | n.d. | n.d. |
BnaCPK30 | KF169740 | 4 | AtCPK30/At1g74740 | OsCPK9/LOC_Os03g48270.1 | 1641 | 61.9 | 6.62 | 4 | MGNCIACV | Yes | Yes |
BnaCPK32 | KF169741 | 4 | AtCPK32/At3g57530 | OsCPK20/LOC_Os07g38120.1 | 1617 | 60.9 | 6.17 | 4 | MGNCCGTA | Yes | Yes |
BnaCPK34 | KC414030 | 10 | AtCPK34/At5g19360 | OsCPK2/LOC_Os01g59360.1 | 1458 | 54 | 5.28 | 4 | MGNCCGRD | Yes | n.d. |