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Table 1 Identification, expression pattern, and functional characterization of differentially secreted proteins in Cochliobolus lunatus at different temperature regimes

From: Secretome weaponries of Cochliobolus lunatusinteracting with potato leaf at different temperature regimes reveal a CL[xxxx]LHM - motif

Spot # aDiff exp. Protein hits bTh. p I/cExp. pI dTh. Mr/eEx. Mr. / fNet Char. gM. Sc. hSeq. Cover. Putative function Interaction conditions
1 Up-regulated 123093|e_gw1.16.937.1 5.7/6.5 39.6/18.2/-4 182 49 Protein kinase activity No potato leaf at 20°C
2 Up-regulated 16455|fgenesh1_pg.2_#_314 9.7/6.5 64.0/15.6/14 179 56 Modulate transcription
3 Up-regulated 118437|e_gw1.13.184.1 10.7/6.6 18.8/11.87/18 398 45 Unknown
4 Up-regulated 125544|e_gw1.19.437.1 7.2/5.5 58.7/42.4/4 365 46 Oxido-reductase activity  
5 Up-regulated 24606|fgenesh1_pg.19_#_39 10.2/5.6 110.2/45.4/46 169 43 Protein kinase activity
6 Up-regulated 19257|fgenesh1_pg.7_#_229 6.2/5.8 195.0/46.3/-1.5 299 33 Unknown
7 Up-regulated 115068|e_gw1.10.331.1 4.2/6.0 261.8/45.2/-157 294 23 Heat shock protein 70kD
8 Up-regulated 129342|estExt_Genewise1.C_1_t20028 6.7/6.2 195.7/36.4/9 179 38 RNA binding binding protein
9 Up-regulated 115068|e_gw1.10.331.1 4.5/5.3 261.8/35.1/-157 279 31 HSP70
10 Up-regulated 129342|estExt_Genewise1.C_1_t20028 6.7/5.4 195.7/32.3/9 110 43 RNA processing
11 Up-regulated 19962|fgenesh1_pg.8_#_384 6.3/5.9 181.8/31.5/1 189 29 Protein kinase activity
12 Up-regulated 116559|e_gw1.11.1289.1 6.7/5.6 114.9/24.3/7.5 275 25 Subtilisin, proteolysis
13 Up-regulated 21721|fgenesh1_pg.12_#_162 9/5.6 146.7/21.4/24 161 26 Pleckstrin-like, intracellular signalling
14 Up-regulated 22022|fgenesh1_pg.12_#_463 10.4/6.8 27.5/16.0/18.5 382 32 Ribonuclease activity
15 Up-regulated 116337|e_gw1.11.1021.1 10.1/6.1 183.2/22.8/59 497 40 Gelsolin-like intratrafficking protein
16 Up-regulated 51954|fgenesh1_pm.10_#_434 10.1/6.1 63.9/16.0/20.5 340 40 DNA binding protein
17 Up-regulated 140405|estExt_Genewise1.C_210317 6.2/6.3 91.9/12.8/91.9 245 28 Arrestin-like signaling protein
18 Up-regulated 137106|estExt_Genewise1.C_13_t10496 4.9/5.9 126.1/14.53/-31.5 198 32 Tetratricopeptide intracellular trafficking
19 Up-regulated 46252|estExt_Genemark1.C_210087 10.0/5.7 52.3/12.3/20.5 287 22 Unknown
21 Up-regulated 64448|estExt_fgenesh1_pg.C_160063 9.9/5.3 112.8/13.1/53 111 29 Unknown
22 Up-regulated 59412|estExt_fgenesh1_pm.C_130013 7.2/5.3 109.9/13.4/12.5 100 34 WD40 repeat, signal transduction
23 Up-regulated 29439|fgenesh1_kg.13_#_117_#_Contig_2 7.1/5.0 28.6/10.7/3 276 39 Short-chain dehydrogenase/reductase
24 Up-regulated 58690|estExt_fgenesh1_pm.C_9_t10419 5.2/5.4 88.5/11.3/-14 369 21 Proteolysis
25 Up-regulated 125654|e_gw1.19.739.1 5.7/5.0 145.5/13.4/-9.5 476 24 Signal transduction response regulator
27 Up-regulated 132047|estExt_Genewise1.C_4_t40098 9.4/4.6 44.7/71.0/6.5 282 49 Unknown Potato leaf at 20°C
28 Induced 123093|e_gw1.16.937.1 5.7/4.4 39.6/18.3/-4 165 21 Protein kinase activity
29 Induced 16455|fgenesh1_pg.2_#_314 9.7/4.2 64.0/14.1/14 186 38 DNA binding transcription factor
30 Induced 114571|e_gw1.10.1404.1 6.6/6.5 49.0/29.0/4 188 22 Carbohydrate metabolism
31 Induced 141118|estExt_Genewise1Plus.C_1_t20192 4.7/6.5 89.9/35.3/-26.5 359 28 Nucleic acid metabolism
32 Induced 44339|estExt_Genemark1.C_10_t10268 5.9/4.5 106.7/20.63/-6.5 162 29 Carbohydrate metabolism
38 Induced 46026|estExt_Genemark1.C_180273 11.2/6.2 21.8/18.6/1.5 195 31 Cytochrome C oxidase Potato leaf at 38°C
  1. #Spot number corresponds to the spots in Additional file 3: Figure S1, Figures 5 and 6. aDifferential protein expression, bTheoretical isoelectric point (pI), cExperimental pI, dTheoretical molecular mass based on whole protein sequence, eExperimental molecular mass of protein subunits in KDa, fPredicted protein net charge based on whole protein sequence, gProtein score reported by MASCOT MS Ion search, and hAmino acid sequence coverage. This group of proteins was used for motif searching by in silico tools. All the proteins were identified by homology search at http://genome.jgi.doe.gov/Coclu2/Coclu2.home.html.