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Table 2 The building process of half-smooth tongue sole physical map with 30,294 BAC clones

From: A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome

Assembly Steps

Contigs

Singletons

Physical length(Mb)

Genome coverage

Q-contigs/Q-clones

Longest contig(kb)

Avr. contig length(kb)

NO. of contigs in different sizes

        

≥ 100

99-50

49-25

24-10

9-3

=2

Initial 1e-60

4200

8662

980

1.56

145/234

958

233

0

1

28

493

2312

1366

DQer (1e-60 to 1e-87)

4260

8807

989

1.58

106/133

958

232

0

0

27

476

2365

1392

Merge 1e-55, 1

3792

7445

960

1.53

106/133

1030

253

0

3

40

604

2252

893

Merge 1e-45, 1

2845

5219

894

1.43

105/133

1465

314

1

7

140

742

1598

357

Merge 1e-35, 2

2592

3356

879

1.40

103/133

1465

339

1

11

198

779

1355

248

Merge 1e-25, 2

2022

1759

838

1.34

102/133

2221

414

4

46

267

735

844

126

Merge 1e-15, 2

1485

585

797

1.27

101/133

3481

539

9

83

311

598

427

57

  1. Note: Contig assembly was performed with the tolerance of 4 and the initial cutoff value of 1e-60, followed by iteration of the end-merge, and singleton-merge routines by means of FPC v9.4. Additional end-merge and singleton-merge routines at 1e-40, 1e-30 and 1e-20 are not shown.