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Table 4 Assessment of the reliability of randomly selected 21 contigs with PCR assays

From: A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome

Contig ID Physical lengths (kb) Number of clones Number of positive clones Proportion of positive clones Number of primer pairs
724 360 18 18 100% 3
2,113 383 17 17 100% 3
27 309 16 16 100% 2
2,259 307 13 13 100% 2
9 499 28 28 100% 2
17 410 19 19 100% 2
85 479 20 20 100% 2
122 352 21 21 100% 2
195 501 42 42 100% 3
148 431 16 16 100% 2
172 397 34 34 100% 2
14 396 43 43 100% 4
1,458 462 27 27 100% 3
52 617 34 34 100% 3
175 704 51 51 100% 2
996 329 43 43 100% 1
252 503 26 26 100% 4
143 941 63 62 98.4% 7
113 1,276 76 76 100% 9
451 1,135 52 52 100% 5
26 360 17 17 100% 2
Total 11,128 676 675 99.9% 65
Average ~530 ~32 ~32   ~3