Table 4 Assessment of the reliability of randomly selected 21 contigs with PCR assays
Contig ID | Physical lengths (kb) | Number of clones | Number of positive clones | Proportion of positive clones | Number of primer pairs |
|---|---|---|---|---|---|
724 | 360 | 18 | 18 | 100% | 3 |
2,113 | 383 | 17 | 17 | 100% | 3 |
27 | 309 | 16 | 16 | 100% | 2 |
2,259 | 307 | 13 | 13 | 100% | 2 |
9 | 499 | 28 | 28 | 100% | 2 |
17 | 410 | 19 | 19 | 100% | 2 |
85 | 479 | 20 | 20 | 100% | 2 |
122 | 352 | 21 | 21 | 100% | 2 |
195 | 501 | 42 | 42 | 100% | 3 |
148 | 431 | 16 | 16 | 100% | 2 |
172 | 397 | 34 | 34 | 100% | 2 |
14 | 396 | 43 | 43 | 100% | 4 |
1,458 | 462 | 27 | 27 | 100% | 3 |
52 | 617 | 34 | 34 | 100% | 3 |
175 | 704 | 51 | 51 | 100% | 2 |
996 | 329 | 43 | 43 | 100% | 1 |
252 | 503 | 26 | 26 | 100% | 4 |
143 | 941 | 63 | 62 | 98.4% | 7 |
113 | 1,276 | 76 | 76 | 100% | 9 |
451 | 1,135 | 52 | 52 | 100% | 5 |
26 | 360 | 17 | 17 | 100% | 2 |
Total | 11,128 | 676 | 675 | 99.9% | 65 |
Average | ~530 | ~32 | ~32 | Â | ~3 |