Figure 2From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomesDetection of ADH activity in X. laevis tissues. Starch gel electrophoresis of tissue homogenates (15 μl) from different animals. (A) ADH1 or ADH1-like activity staining using 2-buten-1-ol as a substrate and NAD+ as a coenzyme. (B) Glutathione (GSH)-dependent formaldehyde dehydrogenase (ADH3) activity staining. Lanes: S, stomach; L, liver; O, ovary (pool of oocytes at different maturation stages). All detected ADH forms showed anodic mobility and different band patterns. ADH1 or ADH1-like activity is more abundant in liver extracts than in stomach and is absent in ovary, whereas ADH3 is more abundant in ovary.Back to article page