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Table 5 Substrate and coenzyme-interacting residues in amphibian ADHs

From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomes

Enzyme Substrate-binding Coenzyme-binding
Inner Middle Outer          
48 93 140 141 57 116 294 318 110 306 309 47 48 51 223 224 225 269 271 363
 Xt-1A S F F V M I V L V M L R S H D T N I N H
 Xl-1A1 S F F V I I - - M - - R S H D T N - - -
 Xl-1A2 S F F M F L V L M M L G S T D T N V N H
 Rp-1 S F F I L I L V L L L R S H D L N I N R
 Xl-1B S F F L I I L L L M L R S H D T N I N R
 Xt-1B S F F L I I L L L M L R S H D T N I N R
 Xl-1C T S F V V F L L I M L H T H D T N V D N
 Xt-1C T S F V V F L L I M L H T H D T N V D T
 Xt-2 T Y F M F A V F L F L R T H D I N I I R
 Xl-3 T Y F M D I V A L F V H T Y D L N I N H
 Xt-3 T Y F M D I V A L F V H T Y D L N I N H
 Xt-7 T C F L L L E I C I F R T H D I N I N R
 Xl-8A S F Y M L F V A L G L G S S G S Q T Y K
 Xt-8A S F F M L F V A V G L G S S G S H T Y Q
 Rp-8 S F L V I M L V F L L G S S G T H A R S
 Xl-8B T C F L L F V P L G M G T A G S H S N A
 Xt-8B T C F V L F V P L G M G T A G S H S N A
 Xl-9 T C F M M V V F F M L H T H D I N V K R
 Xt-9 T C F M M V V F F I L H T H D I N V N R
 Xl-10A S F F L L L V V I M L R S H D V N V H H
 Xt-10A S F F M L M V V M M L R S H D I N V H R
 Xl-10B S V F L F L V M R M L R S H D V N V H L
 Xt-10B S V F M F L A S R M L R S H D I N V H H
  1. All available sequences, full-length and partial, are considered. Sequence annotation consists of source organism abbreviation (Rp: Rana perezi, Xl: Xenopus laevis, Xt: Xenopus tropicalis) followed by the assigned class number. Position numbering is based on horse ADH1E. Residues at positions 223–225 (in bold) are involved in the interaction with the extra phosphate group of NADP+ in ADH8 and determine preference for this coenzyme [19].