|
Enzyme
|
Substrate-binding
|
Coenzyme-binding
|
|---|
|
Inner
|
Middle
|
Outer
| | | | | | | | | |
|---|
|
48
|
93
|
140
|
141
|
57
|
116
|
294
|
318
|
110
|
306
|
309
|
47
|
48
|
51
|
223
|
224
|
225
|
269
|
271
|
363
|
|---|
|
Xt-1A
|
S
|
F
|
F
|
V
|
M
|
I
|
V
|
L
|
V
|
M
|
L
|
R
|
S
|
H
|
D
|
T
|
N
|
I
|
N
|
H
|
|
Xl-1A1
|
S
|
F
|
F
|
V
|
I
|
I
|
-
|
-
|
M
|
-
|
-
|
R
|
S
|
H
|
D
|
T
|
N
|
-
|
-
|
-
|
|
Xl-1A2
|
S
|
F
|
F
|
M
|
F
|
L
|
V
|
L
|
M
|
M
|
L
|
G
|
S
|
T
|
D
|
T
|
N
|
V
|
N
|
H
|
|
Rp-1
|
S
|
F
|
F
|
I
|
L
|
I
|
L
|
V
|
L
|
L
|
L
|
R
|
S
|
H
|
D
|
L
|
N
|
I
|
N
|
R
|
|
Xl-1B
|
S
|
F
|
F
|
L
|
I
|
I
|
L
|
L
|
L
|
M
|
L
|
R
|
S
|
H
|
D
|
T
|
N
|
I
|
N
|
R
|
|
Xt-1B
|
S
|
F
|
F
|
L
|
I
|
I
|
L
|
L
|
L
|
M
|
L
|
R
|
S
|
H
|
D
|
T
|
N
|
I
|
N
|
R
|
|
Xl-1C
|
T
|
S
|
F
|
V
|
V
|
F
|
L
|
L
|
I
|
M
|
L
|
H
|
T
|
H
|
D
|
T
|
N
|
V
|
D
|
N
|
|
Xt-1C
|
T
|
S
|
F
|
V
|
V
|
F
|
L
|
L
|
I
|
M
|
L
|
H
|
T
|
H
|
D
|
T
|
N
|
V
|
D
|
T
|
|
Xt-2
|
T
|
Y
|
F
|
M
|
F
|
A
|
V
|
F
|
L
|
F
|
L
|
R
|
T
|
H
|
D
|
I
|
N
|
I
|
I
|
R
|
|
Xl-3
|
T
|
Y
|
F
|
M
|
D
|
I
|
V
|
A
|
L
|
F
|
V
|
H
|
T
|
Y
|
D
|
L
|
N
|
I
|
N
|
H
|
|
Xt-3
|
T
|
Y
|
F
|
M
|
D
|
I
|
V
|
A
|
L
|
F
|
V
|
H
|
T
|
Y
|
D
|
L
|
N
|
I
|
N
|
H
|
|
Xt-7
|
T
|
C
|
F
|
L
|
L
|
L
|
E
|
I
|
C
|
I
|
F
|
R
|
T
|
H
|
D
|
I
|
N
|
I
|
N
|
R
|
|
Xl-8A
|
S
|
F
|
Y
|
M
|
L
|
F
|
V
|
A
|
L
|
G
|
L
|
G
|
S
|
S
|
G
|
S
|
Q
|
T
|
Y
|
K
|
|
Xt-8A
|
S
|
F
|
F
|
M
|
L
|
F
|
V
|
A
|
V
|
G
|
L
|
G
|
S
|
S
|
G
|
S
|
H
|
T
|
Y
|
Q
|
|
Rp-8
|
S
|
F
|
L
|
V
|
I
|
M
|
L
|
V
|
F
|
L
|
L
|
G
|
S
|
S
|
G
|
T
|
H
|
A
|
R
|
S
|
|
Xl-8B
|
T
|
C
|
F
|
L
|
L
|
F
|
V
|
P
|
L
|
G
|
M
|
G
|
T
|
A
|
G
|
S
|
H
|
S
|
N
|
A
|
|
Xt-8B
|
T
|
C
|
F
|
V
|
L
|
F
|
V
|
P
|
L
|
G
|
M
|
G
|
T
|
A
|
G
|
S
|
H
|
S
|
N
|
A
|
|
Xl-9
|
T
|
C
|
F
|
M
|
M
|
V
|
V
|
F
|
F
|
M
|
L
|
H
|
T
|
H
|
D
|
I
|
N
|
V
|
K
|
R
|
|
Xt-9
|
T
|
C
|
F
|
M
|
M
|
V
|
V
|
F
|
F
|
I
|
L
|
H
|
T
|
H
|
D
|
I
|
N
|
V
|
N
|
R
|
|
Xl-10A
|
S
|
F
|
F
|
L
|
L
|
L
|
V
|
V
|
I
|
M
|
L
|
R
|
S
|
H
|
D
|
V
|
N
|
V
|
H
|
H
|
|
Xt-10A
|
S
|
F
|
F
|
M
|
L
|
M
|
V
|
V
|
M
|
M
|
L
|
R
|
S
|
H
|
D
|
I
|
N
|
V
|
H
|
R
|
|
Xl-10B
|
S
|
V
|
F
|
L
|
F
|
L
|
V
|
M
|
R
|
M
|
L
|
R
|
S
|
H
|
D
|
V
|
N
|
V
|
H
|
L
|
|
Xt-10B
|
S
|
V
|
F
|
M
|
F
|
L
|
A
|
S
|
R
|
M
|
L
|
R
|
S
|
H
|
D
|
I
|
N
|
V
|
H
|
H
|
- All available sequences, full-length and partial, are considered. Sequence annotation consists of source organism abbreviation (Rp: Rana perezi, Xl: Xenopus laevis, Xt: Xenopus tropicalis) followed by the assigned class number. Position numbering is based on horse ADH1E. Residues at positions 223–225 (in bold) are involved in the interaction with the extra phosphate group of NADP+ in ADH8 and determine preference for this coenzyme [19].
