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Table 5 Substrate and coenzyme-interacting residues in amphibian ADHs

From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomes

Enzyme

Substrate-binding

Coenzyme-binding

Inner

Middle

Outer

         

48

93

140

141

57

116

294

318

110

306

309

47

48

51

223

224

225

269

271

363

 Xt-1A

S

F

F

V

M

I

V

L

V

M

L

R

S

H

D

T

N

I

N

H

 Xl-1A1

S

F

F

V

I

I

-

-

M

-

-

R

S

H

D

T

N

-

-

-

 Xl-1A2

S

F

F

M

F

L

V

L

M

M

L

G

S

T

D

T

N

V

N

H

 Rp-1

S

F

F

I

L

I

L

V

L

L

L

R

S

H

D

L

N

I

N

R

 Xl-1B

S

F

F

L

I

I

L

L

L

M

L

R

S

H

D

T

N

I

N

R

 Xt-1B

S

F

F

L

I

I

L

L

L

M

L

R

S

H

D

T

N

I

N

R

 Xl-1C

T

S

F

V

V

F

L

L

I

M

L

H

T

H

D

T

N

V

D

N

 Xt-1C

T

S

F

V

V

F

L

L

I

M

L

H

T

H

D

T

N

V

D

T

 Xt-2

T

Y

F

M

F

A

V

F

L

F

L

R

T

H

D

I

N

I

I

R

 Xl-3

T

Y

F

M

D

I

V

A

L

F

V

H

T

Y

D

L

N

I

N

H

 Xt-3

T

Y

F

M

D

I

V

A

L

F

V

H

T

Y

D

L

N

I

N

H

 Xt-7

T

C

F

L

L

L

E

I

C

I

F

R

T

H

D

I

N

I

N

R

 Xl-8A

S

F

Y

M

L

F

V

A

L

G

L

G

S

S

G

S

Q

T

Y

K

 Xt-8A

S

F

F

M

L

F

V

A

V

G

L

G

S

S

G

S

H

T

Y

Q

 Rp-8

S

F

L

V

I

M

L

V

F

L

L

G

S

S

G

T

H

A

R

S

 Xl-8B

T

C

F

L

L

F

V

P

L

G

M

G

T

A

G

S

H

S

N

A

 Xt-8B

T

C

F

V

L

F

V

P

L

G

M

G

T

A

G

S

H

S

N

A

 Xl-9

T

C

F

M

M

V

V

F

F

M

L

H

T

H

D

I

N

V

K

R

 Xt-9

T

C

F

M

M

V

V

F

F

I

L

H

T

H

D

I

N

V

N

R

 Xl-10A

S

F

F

L

L

L

V

V

I

M

L

R

S

H

D

V

N

V

H

H

 Xt-10A

S

F

F

M

L

M

V

V

M

M

L

R

S

H

D

I

N

V

H

R

 Xl-10B

S

V

F

L

F

L

V

M

R

M

L

R

S

H

D

V

N

V

H

L

 Xt-10B

S

V

F

M

F

L

A

S

R

M

L

R

S

H

D

I

N

V

H

H

  1. All available sequences, full-length and partial, are considered. Sequence annotation consists of source organism abbreviation (Rp: Rana perezi, Xl: Xenopus laevis, Xt: Xenopus tropicalis) followed by the assigned class number. Position numbering is based on horse ADH1E. Residues at positions 223–225 (in bold) are involved in the interaction with the extra phosphate group of NADP+ in ADH8 and determine preference for this coenzyme [19].
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BMC Genomics

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