Figure 2

Comparison of the amino acid sequence of strawberry FaFAD1 with other plant fatty acid desaturases (Δ12-FADs) and hydroxilases (Δ12-FAH). Identical amino acid residues were indicated with black background. Dark and bright gray shade indicated 80% and 60% or more conservation among all the aligned sequences, respectively. The predicted trans-membrane domains (TM-helixes) and highly conserved His boxes are shown. The seven amino acid residues that differ between oleate desaturases and hydroxilases according to [45] (numbering based on the arabidopsis FAD2 sequence) are indicated in green when the amino acid residue is conserved between FaFAD1 and hydroxilases and red when not. Accession numbers for the sequences were as follows: Fragaria x ananassa FaFAD1 (KF887973), Nicotiana tabacum NtFAD (AAT72296), Olea europaea OeFAD2-1 (AAW63040), Davidia involucrate DiFAD2 (ABZ05022), Crepis alpina CaFAD2-2 (ABC00770), Arabidopsis thaliana AtFAD2 (AAA32782), Prunus persica PpFAD1B-6 (AGM53489), Ricinus comunis hydroxylase RcFAH12 (AAC49010), (MDP0000288297), Lesquerella fendleri bifunctional hydroxylase/desaturase LFAH12 (AAC32755).