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Figure 21 | BMC Genomics

Figure 21

From: Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica)

Figure 21

Validation of microarray expression of 12 selected genes by qRT-PCR. A total of 12 genes were selected in the validation using qRT-PCR: (A) Phenylalanine ammonia-lyase (PAL: isotig 10873), (B) Cinnamate-4-hydroxylase (C4H: isotig 09462), (C) 4-Coumarate:CoA ligase (4CL: isotig 04988), (D) Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: isotig 10178), (E) p-Coumarate-3-hydroxylase (C3H: isotig 02271), (F) Caffeoyl-CoA O-methyltransferase; Cinnamyl alcohol dehydrogenase (CCoAOMT: isotig 10196), (G) Cinnamoyl-CoA reductase (CCR: isotig 05079), (H) Cinnamyl alcohol dehydrogenase (CAD: isotig 02638), (I) Sucrose synthase (Sus: isotig 12351), (J) Cellulose synthase (Ces: isotig 08498), (K) α-Tubulin (Tub α: isotig 02753), (L) β−Tubulin (Tub β: isotig 13384). In qRT-PCR, Ubiquitin (UBQ) was used as a reference gene, and the data were calibrated relative to transcript levels in the 24 March sample. Error bars show standard deviation for three replicates.

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