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Table 3 Functional annotations of putative secreted uterine proteins that are up-regulated when a hard-shelled egg is present

From: Hen uterine gene expression profiling during eggshell formation reveals putative proteins involved in the supply of minerals or in the shell mineralization process

Potential functions

Gene symbol (Gene Id)

Gene description

Mineralization Interactions with calcium

SPP1* (395210)

Secreted phosphoprotein 1 (osteopontin), involved in bone mineralization and present in chicken eggshell

PENK* (421131)


COL1A2* (396243)

Collagen, type I, alpha 2

DCN** (417892)

Decorin, leucine-rich repeats (LRRs) participate in protein-protein interactions

SPARCL1** (422586)

SPARC-like 1 (hevin), extracellular Ca2+ binding domain (containing 2 EF-hand motifs), serine protease inhibitor

CD34** (419856)

CD34 molecule, podocalyxin, sialoprotein highly negatively charged

CRELD2** (417735)

Cysteine-rich with calcium binding EGF-like domains 2

MCFD2** (421413)

Multiple coagulation factor deficiency 2, contains calcium-binding EF-hand

MATN2*** (426584)

Matrilin 2, calcium-binding EGF-like domain, mediate cell-matrix and matrix-matrix interactions in cartilaginous


SLIT2* (373967)

Slit homolog 2 (Drosophila), developmental biology with EGF domains includng calcium binding domains


HS6ST2* (395150)

Heparan sulfate 6-O-sulfotransferase 2

EXT1*** (420283)

Exostosin 1, involved in glycosaminoglycans biosynthesis

MGAT4B** (416285)

Mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B, N-Glycan biosynthesis

TSKU* (419088)

Tsukushi small leucine rich proteoglycan homolog, protein-protein interactions

GPC1* (424770)

Glypican 1


ADAMTS1** (418479)

ADAM metallopeptidase with thrombospondin type 1 motif, 1

Molecular chaperone

CLU* (395722)

Clusterin, molecular chaperone

GKN2-OCX21* (419515)

Gastrokine 2 (ovocalyxin-21), eggshell specific protein, brichos domain, molecular chaperone

HSPA5* (396487)

Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa), chaperone help to fold many proteins

HSPA13** (418469)

Heat shock protein 70 kDa family, member 13, molecular chaperone

HSP90B1** (374163)

Endoplasmin, heat shock protein 90 kDa beta (Grp94), member 1, molecular chaperone

HYOU1* (428251)

Hypoxia up-regulated 1, HSP protein, molecular chaperone

ERLEC1* (421220)

Endoplasmic reticulum lectin 1

DNAJB9** (417783)

DnaJ/Hsp40 (heat shock protein 40) proteins, crucial roles in protein translation, folding, unfolding, translocation, and degradation, molecular chaperone

FICD** (416889)

FIC domain containing tetratricopeptide repeat domain involved in variety of functions including protein-protein interactions, molecular chaperone


GRIP2** (416043)

Glutamate receptor interacting protein 2, involved in protein-protein interactions

Antimicrobial proteins

OCX36* (419289)

Ovocalyxin-36, LBP/BPI protein, antimicrobial

BPIL3** (419290)

Bactericidal/permeability-increasing protein-like 3, LBP/BPI protein, antimicrobial

LY86* (420872)

Lymphocyte antigen 86, involved in innate immune response and binding to bacterial LPS

PTN* (418125)

Pleiotrophin, heparin binding protein, antimicrobial

SEMA3C* (374090)

Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C, developmental biology


LOC422316** (422316)

Vascular endothelial growth factor receptor kdr-like, IG-like domain


PROC** (395085)

Protein C (inactivator of coagulation factors), trypsin-like serine protease with EGF-like calcium binding domain

Other roles

P4HA1** (423704)

Prolyl 4-hydroxylase, alpha polypeptide I

SPON1* (395657)

Spondin 1, extracellular matrix protein involved in patterning axonal growth trajectory

POMC* (422011)


SEMA6D* (415430)

Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, involved in the development of the nervous system and in axonal guidance

P4HB* (374091)

Prolyl 4-hydroxylase, beta polypeptide, containing thiredoxin domain

PRDX4* (418601)

Peroxiredoxin 4

FAM3C* (417758)

Family with sequence similarity 3, member C

LOC776741** (776741)

Uncharacterized LOC776741

RET** (396107)

Ret proto-oncogene, cadherin repeat-like domain involved in cell-cell contact when bound to calcium

GREM1** (395826)

Gremlin 1, extracellular DAN domain

WNT11** (395562)

Wingless-type MMTV integration site family, member 11

ROS1** (396192)

C-ros oncogene 1 receptor tyrosine kinase, contains Fibronectin type 3 and protein Tyrosine Kinase domains

ERP29** (416882)

Endoplasmic reticulum protein 29, thioredoxin domain, oxidoreductase

COMT** (416783)


CCDC80** (395074)

Coiled-coil domain containing 80 involved in cell adhesion;

PDGFD** (418978)

Platelet derived growth factor D, biology of development

ERO1LB** (421509)

ERO1-like beta (S. cerevisiae)

PDIA6** (421940)

Protein disulfide isomerase family A, member 6, contains thioredoxin domain

MANF** (770664)

Mesencephalic astrocyte-derived neurotrophic factor, RNA binding protein

CYP26A1** (408183)

Cytochrome P450, family 26, subfamily A, polypeptide 1 involved in the oxidative degradation of various compound

AGR2** (420596)

Anterior gradient homolog 2 (Xenopus laevis), containing Thiroedoxin domain

TNFRSF6B** (395096)

Tumor necrosis factor receptor superfamily domain, involved in nvolved in inflammation response, apoptosis, autoimmunity and organogenesis

PCDH19** (422263)

Protocadherin 19, Cadherin tandem repeat domain involved in Ca2 + −mediated cell-cell adhesion


PDIA3 ** (373899)

Protein disulfide isomerase family A, member 3, containing thiroedoxin domain

  1. *Predicted to be secreted and already identified in the eggshell.
  2. **Predicted to be secreted but not previously identified in the eggshell.
  3. ***Proteins already identified in the eggshell, but not predicted to be secreted.