Figure 3

Average expression stability (M) of remaining reference genes during stepwise exclusion of the least stable reference gene [9] and pairwise variation (V2/V3) of validated miRNAs. I. mir-46-3p, mir-47-3p and mir-2-5p are stable in all conditions tested, II. mir-57-5p, mir-55-3p and mir357-3p are stable upon UV treatment, III. mir-64-5p, mir-86-5p and mir-794-5p are stable upon heat shock, IV. mir-58-3p, mir-230-3p and mir-357-3p are stable upon hypoxia regardless of HIF-1 presence, V. mir-241-3p, mir-254 and mir-52-5p are stable upon hypoxia. VI. Table showing the optimal number of reference miRNAs suggested by V(V2/V3) analysis.