Figure 4

Abundance of validated miRNAs as indicated by the Cq value of qPCR reactions and RNA-seq read counts. I. Graphical representation of the Cq values obtained by qPCR in the control sample. Each box represents the range of Cq values of all the qPCR reactions performed for each miRNA. The horizontal lines in the boxes indicate the median Cq. II. Graphical representation of the RNA-seq read results for each miRNA. In order for the graphs to be in line with the data presentation on the panel I and therefore easily comparable, the number of reads was calculated as a fraction of 1 and the outcome was multiplied by a large number (10⁴) to give a high value. The bigger the number, the smaller the abundance of the miRNA, similar to Cq function.