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Table 1 Summary of the transcriptome sequencing, assembly and enzyme discovery results

From: Peroxidase gene discovery from the horseradish transcriptome

  Number of sequences % of total
Transcriptome sequencing and assembly
Total number of reads 592,507 -
Clipped reads 556,269 -
Reads after Newbler quality control 543,439 100.0
Reads aligned 490,285 90.2
Reads assembled 433,179 88.4
Reads partially assembled 57,064 10.5
Singletons 35,950 7.3
Contigs 18,511 -
Isogroups 10,619 -
Isotigs 14,871 -
Average isotig length 1,133 -
Largest isotig length 3,659 -
Average contig coverage 11.4 -
HRP isoenzyme discovery and verification
# of isotigs with a secretory peroxidase domain 18 -
# of full length peroxidase genes 18 -
# of peroxidase genes after manual revision 20 -
Total # of isoenzymes (including allelic variants) 28 100
Successfully verified from gDNA 26 89.7
Enzyme production in Pichia pastoris
Synthetic genes for production 26 89.7
Successful production of an active isoenzyme 22 75.9