Table 2 Comparison of the HRP isoenzyme sequences between GenBank, UniProt, transcriptome and verified genome sequences
From: Peroxidase gene discovery from the horseradish transcriptome
| HRP | Sanger sequence | Transcriptome sequence | GenBank sequence | UniProt sequence | |||
|---|---|---|---|---|---|---|---|
| Nt | aa (exon)/intron | nt | aa | nt | aa (exon)/intron | aa | |
| C1A | TA | Y37 | - | - | AT | I37 | Y37 |
| 109-110 | 109-110 | ||||||
| C1159 | intron | - | intron | G1159 | intron | - | |
| C1B | T/C253 | intron | - | intron | T253 | intron | - |
| T/C859 | intron | - | intron | C859 | intron | - | |
| C1C | ss | ss | ss | ss | * | * | * |
| nt1-60 | aa1-20 | nt1-60 | aa1-20 | ||||
| C178 | R60 | C178 | R60 | A118 | S40 | S40 | |
| A/T1335 | intron | - | intron | - | - | - | |
| A/G1888 | T/A165 | A/G493 | T/A165 | G433 | A145 | A145 | |
| C1889 | A165 | C/T1889 | A/V165 | ||||
| C/G1921 | Q/E176 | C/G526 | Q/E176 | G466 | E156 | E156 | |
| C2 | CT | intron | - | intron | * | intron | - |
| 1250-1251 | |||||||
| A1334 | intron | - | intron | * | intron | - | |
| C3 | G/T1294 | intron | - | intron | G1294 | intron | - |
| A/T1323 | intron | - | intron | A1323 | intron | - | |
| T/C1484 | L231 | - | - | T1484 | L231 | L231 | |
| C/T1541 | F250 | - | - | C1541 | F250 | F250 | |
| A2 | ss | ss | ss | ss | - | - | * |
| nt1-93 | aa1-31 | 1-93 | aa1-31 | ||||
| AAT | N78 | AAT | N47 | - | - | D47 | |
| 231-234 | 231-234 | ||||||
| GGA | G220 | GGA | G220 | - | - | N189 | |
| 996-998 | 661-663 | ||||||
| AAT | N221 | AAT | N221 | - | - | G190 | |
| 999-1001 | 664-666 | ||||||
| ACG | T284 | ACG | T284 | - | - | L253 | |
| 1185-1187 | 850-852 | ||||||
| G/A1203 | A/T290 | G868 | A290 | - | - | A259 | |
| AAT | N334 | AAT | N334 | - | - | D303 | |
| 1335-1337 | 999-1002 | ||||||
| E5 | ss | ss | ss | ss | - | - | * |
| nt1-81 | aa1-27 | nt1-81 | aa1-27 | ||||
| T419 | L82 | C/T246 | L82 | - | - | L55 | |
| C422 | D83 | T/C249 | D83 | - | - | D56 | |
| C545 | C124 | T/C372 | C124 | - | - | C97 | |
| 01805 | None | none | none | none | - | - | - |
| 22684 | G1611 | R337 | A1010 | K337 | - | - | - |
| TGA | D343 | CGG | G343 | - | - | - | |
| 1627-1629 | 1026-1028 | ||||||
| 01350 | None | none | none | none | - | - | - |
| 02021 | None | none | none | none | - | - | - |
| 03523 | None | none | none | none | - | - | - |
| 06117 | T30 | V10 | C/T30 | V10 | - | - | - |
| C1088 | I269 | T807 | I269 | - | - | - | |
| 17517 | T190 | Y64 | C190 | H64 | - | - | - |
| C1157 | G282 | T846 | G282 | - | - | - | |
| A1232 | K307 | G921 | K307 | - | - | - | |
| 08562.1 | None | none | none | none | - | - | - |
| 08562.4 | None | none | none | none | - | - | - |
| 23190 | T1345 | S109 | G1345 | S109 | - | - | - |
| C1423 | G135 | T1423 | G135 | - | - | - | |
| T1842 | S222 | T/C1842 | S/P222 | ||||
| C1850 | T224 | A/C1850 | T224 | - | - | - | |
| A2221 | E348 | T/A2221 | V/E348 | - | - | - | |
| 04663 | None | none | none | none | - | - | - |
| 06351 | None | none | none | none | - | - | - |
| 05508 | G/A346 | A/T116 | G/A346 | A/T116 | - | - | - |
| 22489 | - | - | G/A597 | T199 | - | - | - |
| . | . | G/T715 | A/S239 | - | - | - | |