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Table 4 Summary of isoenzyme expression and characterization

From: Peroxidase gene discovery from the horseradish transcriptome

Contig number Name (* previously unknown) Activity ABTS Activity TMB Activity guaiacol Activity pyrogallol
- C1A + + + +
15901 C1B - + - -
25148 C1C* + + (+) +
25148_2 C1D* + + (+) +
04627 C2 + + + +
- C3 + + + +
Manual assembly A2A* + + + +
Manual assembly A2B* + + (+) (+)
04382 E5 + + + +
01805 01805* (B1?) + + - -
22684 22684.1* (B2A?) + + - -
22684_2 22684.2* (B2B?) + + - -
01350 01350* + + - -
02021 02021* - - - -
23190 23190.1* - - - -
23190_2 23190.2* n.d. n.d. n.d. n.d.
04663 04663.1* + (+) - -
06351 06351* + + + +
03523 03523* - - - -
05508 05508.1* + + + +
05508_2 05508.2* n.d. n.d. n.d. n.d.
22489_1 22489.1* + + (+) +
22489_2 22489.2* + + (+) +
06117 06117* - - - -
17517_1 17517.1* + - - -
17517_2 17517.2* + + + +
08562_1 08562.1* + + - -
08562_4 08562.2* + + - +
  1. Isoenzymes showing obvious peroxidase activity with the assay used are marked with “+”. Isoenzymes showing very low but detectable peroxidase activity with the assay used are marked with “(+)”. Isoenzymes with no activity detected during an observation period of 2 h are marked with “-“. Allelic variants not produced heterologously are marked with n.d. (no data available). Isoenzymes discovered during this study are marked with “*”.