Transcriptome analysis identifies Bacillus anthracis genes that respond to CO2through an AtxA-dependent mechanism

Table 1 Summary of RNA-sequencing results

Sample ID	Condition	OD600	Total reads	Unique reads	Unique reads (%)	Gene reads	Gene reads (%)
1	WT/CO₂	2.0	62,677,017	35,654,298	57	29,959,488	84
2	WT/CO₂	2.1	63,937,489	40,947,059	64	34,294,534	84
3	ΔAtxA/air	2.0	69,132,947	32,303,226	47	27,284,918	84
4	ΔAtxA/air	1.9	67,144,286	34,755,237	52	30,494,637	88
5	WT/air	2.0	68,812,647	23,082,971	34	17,928,149	78
6	WT/air	2.2	63,046,363	45,881,828	73	39,525,419	86
7	ΔAtxA/CO₂	2.0	60,638,322	39,860,432	66	34,405,268	86
8	ΔAtxA/CO₂	2.3	66,526,879	34,284,152	52	28,722,268	84

OD600 indicates the optical density at which each bacterial sample was subjected to RNA isolation. Total reads represent the number of high-quality reads. Unique reads represents reads that mapped to unique portions of the B. anthracis genome or pXO1 plasmid, excluding ribosomal RNA. Unique reads (%) was calculated as the ratio of unique reads to total reads. Gene reads refers to the number of unique reads that mapped to known protein-coding genes. Gene reads (%) was calculated as the ratio of gene reads to unique reads.

ISSN: 1471-2164