Sample ID
|
Condition
|
OD600
|
Total reads
|
Unique reads
|
Unique reads (%)
|
Gene reads
|
Gene reads (%)
|
---|
1
|
WT/CO2
|
2.0
|
62,677,017
|
35,654,298
|
57
|
29,959,488
|
84
|
2
|
WT/CO2
|
2.1
|
63,937,489
|
40,947,059
|
64
|
34,294,534
|
84
|
3
|
ΔAtxA/air
|
2.0
|
69,132,947
|
32,303,226
|
47
|
27,284,918
|
84
|
4
|
ΔAtxA/air
|
1.9
|
67,144,286
|
34,755,237
|
52
|
30,494,637
|
88
|
5
|
WT/air
|
2.0
|
68,812,647
|
23,082,971
|
34
|
17,928,149
|
78
|
6
|
WT/air
|
2.2
|
63,046,363
|
45,881,828
|
73
|
39,525,419
|
86
|
7
|
ΔAtxA/CO2
|
2.0
|
60,638,322
|
39,860,432
|
66
|
34,405,268
|
86
|
8
|
ΔAtxA/CO2
|
2.3
|
66,526,879
|
34,284,152
|
52
|
28,722,268
|
84
|
- OD600 indicates the optical density at which each bacterial sample was subjected to RNA isolation. Total reads represent the number of high-quality reads. Unique reads represents reads that mapped to unique portions of the B. anthracis genome or pXO1 plasmid, excluding ribosomal RNA. Unique reads (%) was calculated as the ratio of unique reads to total reads. Gene reads refers to the number of unique reads that mapped to known protein-coding genes. Gene reads (%) was calculated as the ratio of gene reads to unique reads.