Transcriptome analysis identifies Bacillus anthracis genes that respond to CO2through an AtxA-dependent mechanism

Table 2 Genes of the virulence condition synergy group

Locus tag	Gene name	Accession number (NCBI)	Adjusted P-Value	Fold-change	Length of predicted protein (AA)	Putative function
pXO1_0122		YP_016453.2	0.0074	5.0	158	Hypothetical protein
	sRNA-1		< 0.0001	338.5		Unknown
pXO1_0123		YP_016454.2	0.0008	68.2	34	Hypothetical protein
pXO1_0124	bslA	YP_016455.2	< 0.0001	192.1	652	Adhesin; virulence factor
pXO1_0125		YP_016456.2	< 0.0001	42.1	280	PAP2 family phosphatase
pXO1_0137		YP_016468.2	0.0008	63.7	151	Hypothetical protein
pXO1_0142	cya	YP_016473.2	0.0065	4.1	800	Edema toxin; virulence factor
	sRNA-2		< 0.0001	332.1		Unknown
pXO1_0151		YP_016482.2	< 0.0001	141.8	44	Hypothetical protein
pXO1_0153		YP_016484.2	< 0.0001	69.1	76	Hypothetical protein
pXO1_0164	pagA	YP_016495.2	< 0.0001	130.9	764	Protective antigen; virulence factor
pXO1_0165		YP_016496.2	< 0.0001	84.2	34	Hypothetical protein
pXO1_0166	pagR	YP_016497.2	< 0.0001	126.2	99	Transcriptional regulator
pXO1_0171		YP_016502.2	< 0.0001	17.7	68	Hypothetical protein
pXO1_0172	lef	YP_016503.2	0.0024	30.9	809	Lethal toxin; virulence factor
GBAA0793		YP_017427.1	0.0002	10.9	436	Phosphotransferase system
GBAA0795		YP_052611.1	<0.0001	9.7	361	DUF871 superfamily
GBAA2301		YP_018949.1	<0.0001	7.5	90	YokU superfamily

This table lists genes whose expression patterns are best explained by a synergistic relationship between the presence of AtxA and CO₂. P-values were determined using a likelihood ratio test of the interaction between genotype and environment. Fold-change refers to the ratio of the counts mapping to that gene in conditions of WT/CO₂ over ΔAtxA/air. Gene functions listed are those determined by validated experiments in B. anthracis or the primary conserved domain is indicated if validated data are lacking. In the absence of conserved domains, the function is listed as a “Hypothetical protein”.

ISSN: 1471-2164