Figure 2
Hierarchical clustering of LAGI02 contigs differentially expressed under phosphate deficiency. A) Heatmap of log10(FPKM + 1) values for the expression of LAGI02 contigs in the different analysed white lupin root tissues. Shown are only the 835 contigs with an higher than 8-fold up- or down-regulation in at least one pair-wise comparison of tissues. Hierarchical clustering using the Manhattan distance identified five main clusters. Clusters 1, 2 and 5 contained genes up-regulated by P-deficiency and more highly expressed in the mature cluster root than in the immature cluster root and the tip. B) Average expression pattern of genes in the main five clusters (means ± SD). In addition, known P-deficiency responsive genes within each cluster are given. For contigs the name of the identified homologous Arabidopsis genes are given. Abbreviation: SPX, SPX DOMAIN; PAP, PURPLE ACID PHOSPHATASE, PHT1, PHOSPHATE TRANSPORTER1; GPX-PDE, GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE; PS, PHOSPHATE STARVATION, PLC, PHOSPHOLIPASE C; SQD, SULFOQUINOVOSYLDIACYLGLYCEROL; MGD, MONOGALACTOSYL DIACYLGLYCEROL SYNTHASE; PPase, PHOSPHORYLASE, PHO2, PHOSPHATE2; PEPC, PHOPSPHOENOLPYRUVATE CARBOXYLASE; PLDζ2, PHOSPHOLIPASEζ2.