Figure 7

Heatmap for pri-miRNA transcript abundance in the five analysed root tissues. Transcript abundance of the identified pri-miRNAs was similar across the analysed root tissues of P-deficient and P-sufficient plants with the exception of pri-miRNA 166 and 393. miRNA156 is 2 to 3-fold more abundant in tissues from P-deficient cluster roots. The pri-miRNA166 is less abundant in the MCR and TCR while pri-miRNA393 was more abundant in the TR and ICR. Although the detection of these pri-miRNAs was indication of their functional role in the regulation, their abundance was not a reliable measure for post-transcriptional regulation of their turn-over and thus degradation of their downstream targets. Targets of the miRNAs are involved in auxin signalling (AFB, AUXIN SIGNALING F-BOX; ARF, AUXIN RESPONSE FACTOR; TIR1, TRANSPORT INHIBITOR RESPONSE1), development (HB2, HOMEOBOX PROTEIN; GRF, GROWTH REGULATING FACTOR; PHB, PHABULOSA; PHV, PHAVOLUTA; SPL, SQUAMOSA PROMOTER BINDING-LIKE) or miRNA processing (AGO1, ARGONAUTE1; DCL1, DICER LIKE1).