Table 1 Bovine specific oligonucleotide forward and reverse primer sequences (5′-3′) and PCR product length

18S/28S

F: 5′- TGCTCTCGCAAACCTAACCT-3′

DQ222453

159

R: 5′- CACTAAGCACTCGCATTCCA-3′

ACTA2

F: 5′- ACTGGGACGACATGGAAAAG -3′

BT021508

166

R: 5′- TACATGGCTGGGACATTGAA-3′

ACTB

F: 5′- ACTTGCGCAGAAAACGAGAT-3′

BT030480

121

R: 5′-CACCTTCACCGTTCCAGTTT-3′

APEH

F: 5′- CAAGAGCATGCGCAGTATGT -3′

BC123400

181

R: 5′- GTAGAGCTGCAAAGCCCATC-3′

CELA1

F: 5′- GGAACCATCCTGGCTAACAA -3′

BC149525

165

R: 5′- CATGGTGGTCTTCACAGTGG -3′

DONSON

F: 5′- TGTGTTGGTGAAGGGAATGA -3′

BC133573.1

107

R: 5′- AGAGGGTTGGTGGAAGTCCT -3′

FST

F: 5′- TAAATGAGAGACCCGCCAAC-3′

AY775795

171

R: 5′- CCCCAGTTTCTGTCCTGTGT-3′

GALNT6

F: 5′- GACCACGTCTTGGACCTCAT-3′

NM_001015534

146

R: 5′- AGCTCAGCTGGGGTGTAGAA-3′

GAPDH

F: 5′- GGGTCATCATCTCTGCACCT-3′

NM_001034034

176

R: 5′- GGTCATAAGTCCCTCCACGA-3′

GJA1

F: 5′- - CAACATGGGTGACTGGAGTG 3′

BT021508

110

R: 5′- GCAGGATTCGGAAAATGAAA -3′

IL33

F: 5′- TTGTTTTGGAGGATGGAAGC -3′

BC123562

163

R: 5′- TTTGTGGGGCTCAGGTTTAC -3′

MMP19

F: 5′- TGGACGTTATCCCCTCAGTC-3′

BC123722

119

R: 5′- GTCCATGGTTCATGCTTGTG -3′

MOSC2

F: 5′- GCAGTGCTTTTGAGGAGGAC-3′

NM_001076380

169

R: 5′- GGATCACACAGGCGGTAACT-3′

NMB

F: 5′- ACATGACGACATGGCTGAAA-3′

NM_001075270

185

R: 5′- ACTTCAACAGGGAAGCGAGA-3′

NPPC

F: 5′- GAGGCAACAAGAAGGGTTTG -3′

BC123399

149

R: 5′- CTGATGACCAAGGGTGACCT -3′

PPARA

F: 5′- TTGTGGCTGCTATCATTTGC-3′

AF229356

135

R: 5′- AGAGGAAGACGTCGTCAGGA-3′

RAB3B

F: 5′- TGGGCGGAGATTCATTTTAC -3′

BC112795

144

R: 5′- GAAAAGTGTGCATGGGTGTG -3′

RPL15

F: 5′- TGCATAAGCACAGGGAGATG-3′

BT020706

134

R: 5′-CTGGAGAGTATTGCGCCTTC-3′

SFRP1

F: 5′- GTCCCTCTGGGTGAATCTGA-3′

NM_174460

158

R: 5′- TCACTAATTGCCAGGGGTTC-3′

SLC1A3

F: 5′-CATCCATGCTGTCATTGTCC-3′

BC120125

188

R: 5′-ATCTGGTAACGCGTTTGTCC -3′

SLC45A2

F: 5′- CATGCCCTCTTCACAGGTTT-3′

XM_001251343

179

R: 5′- AGTGGGGCTTCAGGGATACT -3′

TGFB1I1

F: 5′- CCTGCAATAAACCCATTGCT-3′

NM_001035313

162

R: 5′- AGAAGCGCTCGAAGTAGCAC-3′

UBQ

F: 5′- TACAACAGTTGGTGGCCAAA-3′

BC102888

121

R: 5′-GAAGACTGGGCTGACTGAGG-3′