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Table 2 Efficiency variables for individual RT-qPCR genes

From: Global gene expression in endometrium of high and low fertility heifers during the mid-luteal phase of the estrous cycle

Gene Optimum [primer] μM Slope (−) R2 Efficiency
ACTA2 5 3.62 0.99 1.890
APEH 20 3.18 0.99 2.064
CELA1 10 3.54 0.99 1.917
DONSON 10 3.20 0.99 2.054
FST 2.5 3.60 0.97 1.896
GALNT6 5 3.29 0.99 2.103
GJA1 20 3.31 0.98 2.003
IL33 5 3.33 0.96 1.997
MMP19 10 3.52 0.99 1.925
MOSC2 10 3.14 0.99 2.082
NMB 10 3.21 0.99 2.049
NPPC 5 3.63 0.99 1.885
PPARA 5 3.33 0.99 1.997
RAB3B 20 3.51 0.99 1.927
SFRP1 5 3.70 0.97 1.863
SLC1A3 10 3.46 0.97 1.946
SLC45A2 10 3.54 0.95 1.916
TGFB1I1 20 3.29 0.96 2.013
UBQ 2.5 3.20 0.99 2.054