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Table 1 Organs and tissues used in the RRIM600 and RRIM600 OPS RNA extracts

From: De novo transcriptome analysis of Hevea brasiliensistissues by RNA-seq and screening for molecular markers

RRIM600 RIN* RRIM600 OPS RIN
Apical meristem 3.0 Cotyledon in the seed (germination stage I) 4.7
Leaflets stage A 3.3 Seedlings (germination stage II) 5.3
Leaflets stage B2 1.8 Stalk (germination stage III) 3.7
Leaflets stage C 2.7 Roots (germination stage III) 3.7
Leaflets stage D 2.5 Seed (germination stage III) 3.3
Petiole (Leaf stage B2) 2.1 Leaves (germination stage IV) 1.6
Petiole (Leaf stage C) 2.7 Stalk (germination stage IV) 3.5
Petiole (Leaflets stage D) 2.7 Roots (germination stage IV) 3.4
Lignified stem 3.0 Seed (germination stage IV) 3.3
Bark (trunk grafted with MDF180 crown) 3.1 Immature seeds with transparent endosperm 2.2
Bark (trunk and crown of RRIM600) 2.8 Immature seeds with white endosperm 1.2
Latex 4.1 Fertilized female flowers 4.5
Raceme 2.9   
Male flowers, mature and immature 3.7   
Columns and wall of fruit lobes 3.3   
Fruit peel 3.4   
Peduncle 2.8   
Seed stage A endosperm 1.2   
  1. Leaf development: Stage A: the preformed leaves in the terminal bud appear; Stage B2: upward leaflets of small size are anthocyanic initially. Then, the lamina reverses and bends to the ground and the anthocyanic color diminishes; Stage C: Rapid lamina growth. The flabby and pale green lamina maintains a drooping position; Stage D: leaves straighten into a horizontal position and become stiff; Seed germination. Stage I: appearance of the radicle; Stage II: appearance of the primary root, side roots and stalk; Stage III: elongation of the primary root, side roots and stalk; Stage IV: elongation of the primary root, side roots and growth of the first two branches. *The RIN software algorithm allows classification of total RNA, based on a numbering system from 1 to 10, with 1 being the most degraded profile and 10 being the most intact.