Figure 1

ChIP-seq analysis of AmrZ binding sites in the Pseudomonas fluorescens F113 chromosome. A. Distribution of putative binding sites along the F113 chromosome. Y axe represents the number of readings on each chromosome position. X axe represents the chromosome position with a window of 100 nucleotides. Red line marks the average number of readings plus standard deviation. B. ChIP analysis of selected enriched regions. 18 regions that appeared enriched in the ChIP-seq analysis were tested in an independent ChIP assay by qPCR. Selected regions corresponded to regions upstream of genes or ORFs as indicated in the x axe. Numbers correspond to PSF113 annotation. Y axe represents fold enrichment of immunoprecipitated DNA (black bars) against a mock control (gray bars). The region upstream the fleQ gene, that did not appeared as enriched in the ChIP-seq assay was also included in the analysis. The asterisks denote statistically significant differences with the mock controls (p < 0.05).