Molecular phylogenetic analysis of the caleosin gene families of T. aestivum , H. vulgare , B. distachyon , S. cereale , O. sativa , and A. thaliana amino acid sequences. The evolutionary history was inferred by using the maximum likelihood method based on the Whelan and Goldman model . The tree with the highest log likelihood (−3638.1820) is shown. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The values on the tree represent bootstrap confidence values inferred from 100 replicates. Brachypodium gene identifiers are taken from http://brachypodium.org/; rice IDs are from GenBank and Arabidopsis IDs are from The Arabidopsis Information Resource (TAIR) . Only one of each representative wheat caleosin homeologous groups was used in order to simplify the phylogenetic tree. The relationship among the wheat gene family members is shown in Additional file 5: Figure S2.