Figure 2

The relative level of expression of ten caleosin gene family members measured in a panel of rye tissues. The expression of caleosin gene family members was estimated in 454 cDNA sequence libraries from anther, crown, root and stem rye tissues using RNA-seq analysis. The aligned 454-cDNAs to each caleosin member were counted, then normalized based on gene lengths and library depths using the RPKM method. Values are the total RPKM of two replicates. A one-way ANOVA was carried out to test the significance of the differences in caleosin gene expression in each rye tissue. Duncan’s multiple range test was used to determine the significant differences in caleosin gene expression in each tissue. Rankings determined by Duncan’s test (p ≤ 0.05), are denoted by different letters, and are indicated on each bar in the graph.