A comparison of Clo gene expression in rye and triticale, to assay the changes in gene expression as a result of polyploidization. The expression of caleosin gene family members was measured using RNA-seq analysis in the stem (A), and anther (B), of rye and triticale, respectively. The 454-cDNA sequences aligned to each caleosin member were counted, then normalized based on gene lengths and library depths using the RPKM method. The expression of each paralog is subdivided into expression of each of the three homeologs for triticale (T), visualized as black bars for the R subgenome, grey bars for the B subgenome, and white bars for the A subgenome. The expression of caleosin family members in rye (R) is represented by solid black bars. χ2 contingency tests based on the null hypothesis that the level of individual R genome caleosins in triticale was not different than one third of the level of expression of the genes in rye were carried out. The *marks individual caleosins with p < 0.05, where the null hypothesis was not accepted.