Figure 4

Top ranked canonical pathways generated in Ingenuity Pathway Analysis (A), and enriched GO terms of interest (B), determined by the GOEAST program, for a set of genes 3-fold differentially regulated with a Benjamini Hochberg False Discovery Rate P < 0.05 between small and large follicles. A The bar chart on the left represents the percentage of genes from the data set that map to each canonical pathway showing those which are up regulated (in red) and down regulated (in blue) in large with respect to small follicles. The pathways are ranked from lowest (top) to highest (bottom) degree of association with genes from the data set by the P-value of a right tailed Fishers exact t-test. The Benjamini-Hochberg test for multiple comparisons determined that these pathways all had –log P value = 0.23. B The bar chart on the left represents the percentage of genes differentially regulated from the data set, which map to an enriched GO term of interest classified as a biological process. The most significant terms from the analysis were not displayed as these were too general and not informative in terms of specific function. The GO terms were ranked from lowest to highest degree of association with genes from our data set, by the P-value calculated using the Benjamini-Yuketeli test for multiple comparisons (top to bottom in graph on right).