Skip to main content

Table 1 The major proteins and peptides of the C. nemoralis shell: 59 proteins and peptides (with an iBAQ percentage of more than 0.1) constitute 93% of the identifiable C. nemoralis shell proteome

From: Characterization of the pigmented shell-forming proteome of the common grove snail Cepaea nemoralis

Isotig Similarity to (best match) E-value Identity Protein features1 Fraction Unique + razor peptides % of total (iBAQ)
123 None - - (12% G, 18% P) S > I 15 26.22
1152 None - - (16% G, 15% M, 10% S); SP S > I 3 15.55
7508 None - - SP S < I 15 6.44
1265 None - - (12% G) S > I 9 4.40
2668 Q4LDE5 2.0e-8 32% Domains: Sushi/SCR/CCP; (10% A, 13% G, 10% S); SP S > I 13 3.87
Homo sapiens
21112 None - - (18% P, 10% N) S > I 8 3.41
14344 None - - (17% Q, 10% L, 17% P) S < I 11 2.96
821 J7Q5J6 8.7e-11 29.9% Similar to BSMP; Domains: CBM_14/ CHIT_BIND_II S < I 15 2.45
Patella vulgata
4164 None - - Similarity to UP2_HALAI (e-value 0.19; 28.1% identity) S > I 3 2.07
58150 K1QZ49 1.2e-40 35.1% Similar to adipocyte plasma membrane-associated protein; Domains: TolB_like/ strictosidine synthase; see also contig_221 and contig_16710; SP S < I 16 1.98
Crassostrea gigas
5087 None - - TM S < I 39 1.60
35852 None - - (11% A, 11% P) S > I 14 1.46
12941 None - - (18% D, 14% L) S < I 12 1.37
10584 None - - (16% D, 10% E) S < I 14 1.35
2108 None - - (10% A, 10% Q) S < I 8 1.32
1850 None - - (22% P); SP S > I 15 1.31
201 None - - (11% N, 10% Q); SP S < I 11 1.20
1504 None - - (16% G, 12% L, 21% M, 10% S in 58AA); SP S > I 1 1.15
16878 None - - (29% Q, 16% P) S > I 3 1.08
2744 None - - (10% K, 10% S) S < I 14 0.90
551 None - - (Fragment of 67AA containing 18% G, 22% M, 12% P) S > I 3 0.85
7809 None - - (21% D, 16% L) S < I 17 0.83
25891 J7QJT8 6.3e-25 34.7% Domains: αCA; SP S < I 42 0.59
Patella vulgata
3938 None - - SP S > I 2 0.58
1188 + 4282 K1P9P0 1.2e-27 47.0% Mesenchyme-specific cell surface glycoprotein; Domains:WD40/YVTN repeat-like S < I 15 0.53
Crassostrea gigas 4.4e-21 50.9%
7563 None - - - S,I 6 0.51
6176 None - - (11% A, 11% G, 12% M) S > I 7 0.49
1604 None - - (31% Q, 23% P) S > I 2 0.48
269 None - - (18% G) S < I 8 0.44
14003 K1PRD3 3.6e-22 30.1% Similar to IgGFc-binding protein; Domains: CBM_14/CHIT_BIND_II; shares peptide with 101824 S,I 18 0.38
Crassostrea gigas
1647 None - - Domains:Sushi/SCR/CCP; (12% P, 11% S, 11% T) S > I 9 0.38
450 68CYM6 9.9e-67 74.2% G-type lysozyme;SP S < I 5 0.36
Physella acuta
1323 A7T0W4 1.3e-26 40.5% Domains: polysaccharide deacetylase/chitinase S < I 7 0.32
Nematostella vectensis
101824 K1QJK2 2.1e-22 29.4% Domains: CBM_14/ CHIT_BIND_II S < I 29 0.31
Crassostrea gigas
84589 K1QIK2 5.4e-41 29.2% Domains: CBM_14/ CHIT_BIND_II; also see contig_101824 S < I 29 0.30
Crassostrea gigas
132 None - - (12% Q) S < I 21 0.29
28994* K1QPM9 1.5e-22 45.6% Similar to fatty acid-binding protein, brain S,I 7 0.24
Crassostrea gigas
32297 None - - (14% G, 10% P); SP S > I 2 0.23
74063 None - - (10% G, 13% L, 20% P); SP S,I 1 0.22
263 None - - - S < I 1 0.21
63304 K1Q365 9.6e-55 29.8% AA 127–818 similar to lactadherin; Domains: CBM_14/CHIT_BIND_II S < I 26 0.21
Crassostrea gigas
227 D3BGG3 8.1e-5 23.9% Similar to Zipper-like Domains-containing protein S < I 14 0.20
Polysphondylium pallidum
691 A5Z1D6 1.4e-89 43.3% Similar to epiphragmin; Domains: Fibr_C; SP S < I 31 0.20
Cernuella virgata
2357 None - - AA 395–528 similar to Domains: LDL_recept_a; (15% T); SP S,I 12 0.19
572* IFEA 1.7e-118 96.9% Non-neuronal cytoplasmic intermediate filament protein S < I 32 0.19
Helix aspersa
7323 A7RQD5 6.2e-41 32.2% SP S < I 25 0.19
Nematostella vectensis
3883 K1Q9V3 5.9e-190 86.2% V-type proton ATPase catalytic subunit A S < I 27 0.17
Crassostrea gigas
943* G0ZGZ8 5.2e-38 97.8% Actin S < I 3 0.16
Metaphire posthuma
169764 R7VB66 3.0e-31 54.5% SP S < I 6 0.16
Capitella telata
104312 R7TB34 2.4e-5 45.5% Similar to α-carbonic anhydrase; Domains: α-CA_2 S,I 4 0.14
Capitella telata
196388 None - - (12% A, 11% G, 11% P, 14% S); S < I 1 0.14
2858 H2ZUY5 5.5e-41 44.6% Similar to adipocyte plasma membrane-associated protein; Domains: TolB_like/strictosidine:synthase_related S < I 11 0.14
Latimeria chalumnae
248122 C3Z1I6 6.1e-15 38.8% Similar to chitinase; Domains: glyco_hydro_18/chitinase S < I 4 0.13
Branchiostoma floridae
7807 None - - Domains: WAP; (12% A, 11% Q); SP S < I 15 0.12
1237 K1QI28 1.9e-191 89.8% V-type proton ATPase subunit B S < I 18 0.11
Crassostrea gigas
20308 Q2LZN0 1.6e-9 27.0% AA 49–467 similar to Dpse/GA10422/alkaline phosphatase; Domains: alkaline phosphatase; SP S < I 14 0.11
Drosophila pseudoobscura
20360 H9K6W1 3.9e-37 37.5% Similar to cadherin; Domains: cadherin; see also contig_75801 S < I 8 0.11
Apis mellifera
31170 K7S1Q8 3.2e-37 35.0% Domains: CMB_14/CHIT_BIND_II; SP S < I 17 0.11
Propionibacterium acidipropionici
  1. All proteins listed here were found in all pigment fractions (yellow, orange and dark brown) with the exception of isotig_169764 which was only found in yellow and orange fractions. Proteins and peptides are listed in order of abundance expressed as a percentage of the shell total proteome that we can identify.
  2. 1The percentages of particularly abundant amino acid residues are given in brackets. “SP” indicates the presence of a signal peptide, “TM” indicates a likely trans-membrane protein.